PXD012000

PXD012000 is an original dataset announced via ProteomeXchange.

Title	Clinical proteomics of invasive breast carcinoma reveals a major role for proline metabolism in imparting drug resistance
Description	Aggressive breast tumors are routinely treated with pre-operative chemotherapy. However, a subset of patients have recurrence despite adjuvant treatment. To identify metabolic processes involved in drug resistance, we took a mass spectrometry-based proteomic approach, and analyzed a breast cancer cohort of 113 samples comprising of breast tumors before and after chemotherapy, with matched tumor adjacent normal tissue from partial responders that underwent neoadjuvant treatment (NAT). Pattern analysis of 7180 proteins revealed more than 1000 proteins with significantly differential expression in primary tumor relative to the healthy tissue, which do not respond to treatment, in treatment resistant patients. Among those, we found significant upregulation of the proline biosynthesis pathway, primarily, PYCR1 that significantly correlated with lower recurrence free survival time in our cohort. Functional analysis showed that PYCR1 induced a pro-survival effect upon treatment with chemotherapy drugs thus emphasizing the potential role of PYCR1 in drug resistance in advanced breast cancer.
HostingRepository	PRIDE
AnnounceDate	2020-08-24
AnnouncementXML	Submission_2020-08-24_00:46:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Geiger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-10 08:26:12	ID requested
⏵ 1	2020-08-24 00:46:43	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Clinical proteomics, Neoadjuvant chemotherapy, Proline biosynthesis, Metabolism

Tamar Geiger
contact affiliation	Department of Human Molecular Genetics and Biochemistry Sackler Faculty of Medicine, Tel Aviv University Israel
contact email	geiger@tauex.tau.ac.il
lab head
Tamar Geiger
contact affiliation	Tel Aviv University
contact email	geiger@tauex.tau.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD012000

PRIDE project URI

• PRIDE
  1. PXD012000
     1. Label: PRIDE project
     2. Name: Clinical proteomics of invasive breast carcinoma reveals a major role for proline metabolism in imparting drug resistance