PXD011998

PXD011998 is an original dataset announced via ProteomeXchange.

Title	Using SWATH-MS to unveil hCSCs response to hCM I/R injury paracrine factors
Description	Human Cardiac/Progenitod Stem Cells (hCSCs) transplantation is arising as a novel therapy option for acute myocardial infarction (AMI) patients. The majority of the studies reported point to paracrine signaling as the main effector in hCSCs activation and regenerative capacity. However, the mechanisms of action of these cells in response to AMI paracrine context are still poorly understood. In the present study, a quantitative proteomics analysis (SWATH-MS) was employed to investigate hCSC response to paracrine factors released by human cardiomyocytes (hCMs) in response to an in vitro AMI Ischemia/Reperfusion (I/R) injury. A total of 714 proteins were quantified, from which 86 were differentially expressed, including 63 proteins up-regulated and 23 down-regulated in hCSCs exposed to hCM I/R injury conditioned media vs hCSCs exposed to control hCM conditioned media. Bioinformatics functional analysis revealed up-regulation of proteins involved with endocytosis, paracrine signaling, proliferation, migration and stress response. This work provides an insight into hCSC biology in response to paracrine cues derived from hCMs upon I/R injury, and the data generated herein constitutes a rich resource for further studies aiming at potentiating the regenerative properties of these cells.
HostingRepository	PRIDE
AnnounceDate	2019-09-25
AnnouncementXML	Submission_2019-09-25_13:36:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Sebastião
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-10 08:25:33	ID requested
⏵ 1	2019-09-25 13:36:06	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: human cardiac stem cells, myocardial ischemia/reperfusion injury, acute myocardial infarctio, human induced pluripotent stem cell derived cardiomyocytes.

Paula Marques Alves
contact affiliation	ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157, Oeiras, Portugal; iBET- Instituto de Biologia Experimental e Tecnológica, Apartado 12 2780-901 Oeiras, Portugal
contact email	marques@ibet.pt
lab head
Maria Sebastião
contact affiliation	iBET/ ITQB-NOVA
contact email	msebastiao@itqb.unl.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD011998

PRIDE project URI

• PRIDE
  1. PXD011998
     1. Label: PRIDE project
     2. Name: Using SWATH-MS to unveil hCSCs response to hCM I/R injury paracrine factors