PXD011998 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Using SWATH-MS to unveil hCSCs response to hCM I/R injury paracrine factors |
Description | Human Cardiac/Progenitod Stem Cells (hCSCs) transplantation is arising as a novel therapy option for acute myocardial infarction (AMI) patients. The majority of the studies reported point to paracrine signaling as the main effector in hCSCs activation and regenerative capacity. However, the mechanisms of action of these cells in response to AMI paracrine context are still poorly understood. In the present study, a quantitative proteomics analysis (SWATH-MS) was employed to investigate hCSC response to paracrine factors released by human cardiomyocytes (hCMs) in response to an in vitro AMI Ischemia/Reperfusion (I/R) injury. A total of 714 proteins were quantified, from which 86 were differentially expressed, including 63 proteins up-regulated and 23 down-regulated in hCSCs exposed to hCM I/R injury conditioned media vs hCSCs exposed to control hCM conditioned media. Bioinformatics functional analysis revealed up-regulation of proteins involved with endocytosis, paracrine signaling, proliferation, migration and stress response. This work provides an insight into hCSC biology in response to paracrine cues derived from hCMs upon I/R injury, and the data generated herein constitutes a rich resource for further studies aiming at potentiating the regenerative properties of these cells. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-25 |
AnnouncementXML | Submission_2019-09-25_13:36:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maria Sebastião |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-10 08:25:33 | ID requested | |
⏵ 1 | 2019-09-25 13:36:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: human cardiac stem cells, myocardial ischemia/reperfusion injury, acute myocardial infarctio, human induced pluripotent stem cell derived cardiomyocytes. |
Contact List
Paula Marques Alves |
contact affiliation | ITQB-NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157, Oeiras, Portugal; iBET- Instituto de Biologia Experimental e Tecnológica, Apartado 12 2780-901 Oeiras, Portugal |
contact email | marques@ibet.pt |
lab head | |
Maria Sebastião |
contact affiliation | iBET/ ITQB-NOVA |
contact email | msebastiao@itqb.unl.pt |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011998
- Label: PRIDE project
- Name: Using SWATH-MS to unveil hCSCs response to hCM I/R injury paracrine factors