PXD011997

PXD011997 is an original dataset announced via ProteomeXchange.

Title	Proteomic responses of Candida albicans to Galleria mellonella larvae hemolymph
Description	This study assessed the development of disseminated candidiasis within Galleria mellonella larvae and characterised the proteomic responses of Candida albicans to larval hemolymph. Infection of larvae with an inoculum of 1 × 106 yeast cell reduced larval viability 24 (53.33 ± 3.33%), 48 (33.33 ± 3.33%) and 72 (6.66 ± 3.33%) hour post infection. C. albicans infection quickly disseminated from the site of inoculation and the presence of yeast and hyphal forms were found in nodules extracted from infected larvae at 6 and 24 hours. A range of proteins secreted during infection of G. mellonella were detected in larval hemolymph and these were enriched for biological processes such as interaction with host and pathogenesis. The candicidal activity of hemolymph after immediate incubation of yeast cells resulted in a decrease in yeast cell viability (0.23 ± 0.03 × 106, p < 0.05) as compared to control (0.99 ± 0.01 × 106). extracellular (in vivo) proteome of C. albicans in larval hemolymph were assessed. C. albicans responds to incubation in hemolymph ex vivo by the induction of an oxidative stress response, a decrease in proteins associated with protein synthesis and an increase in glycolytic proteins.
HostingRepository	PRIDE
AnnounceDate	2019-01-17
AnnouncementXML	Submission_2019-01-17_05:01:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerard Sheehan
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-10 08:25:18	ID requested
⏵ 1	2019-01-17 05:01:56	announced

Sheehan G, Kavanagh K, Larvae. J Fungi (Basel), 5(1):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Candida

Galleria, Infection, Immunity, Mini-model, in vivo screening

Prof Kevin Kavanagh
contact affiliation	Medical Mycology Laboratory, Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland.
contact email	kevin.kavanagh@mu.ie
lab head
Gerard Sheehan
contact affiliation	Maynooth University
contact email	gerard.sheehan.2013@mumail.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011997

PRIDE project URI

• PRIDE
  1. PXD011997
     1. Label: PRIDE project
     2. Name: Proteomic responses of Candida albicans to Galleria mellonella larvae hemolymph