PXD011993

PXD011993 is an original dataset announced via ProteomeXchange.

Title	Targeting of precursor polypeptides to the human ER
Description	Various pathways can target nascent or fully synthesized precursor polypeptides to the human endoplasmic reticulum (ER). Typically, they involve cytosolic proteins or complexes and their respective receptors on the ER surface. The signal recognition particle (SRP) and the heterodimeric SRP-receptor (SR) represent one such targeting system, others are TRC40 and its heterodimeric TRC-receptor (WRB/CAML) and the components of the SND pathway. Apparently, they all can target precursor polypeptides to the Sec61-channel in the ER membrane. To characterize the substrate specificities of these targeting pathways, we combined siRNA-mediated depletion of membrane receptor subunits in HeLa cells with label-free quantitative proteomics and differential protein abundance analyis.
HostingRepository	PRIDE
AnnounceDate	2020-03-06
AnnouncementXML	Submission_2020-03-06_04:21:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Zimmermann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-10 03:18:50	ID requested
⏵ 1	2020-03-06 04:21:58	announced

Schorr S, Nguyen D, Ha, ß, denteufel S, Nagaraj N, Cavali, é A, Greiner M, Weissgerber P, Loi M, Paton AW, Paton JC, Molinari M, F, ö, rster F, Dudek J, Lang S, Helms V, Zimmermann R, Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import. FEBS J, 287(21):4612-4640(2020) [pubmed]

curator keyword: Biological

submitter keyword: HeLa, SR, SND2, WRB/CAML

Prof. Dr. Richard Zimmermann (lab head)
contact affiliation	Medizinische Biochemie und Molekularbiologie, Universität des Saarlandes, Gebäude 44, Kirrbergerstr. 100, 66421 Homburg, Deutschland Nagarjuna Nagaraj, Max-Planck-Insitute for Biochemistry, Core facilty (contact)
contact email	richard.zimmermann@uks.eu
lab head
Richard Zimmermann
contact affiliation	Saarland University
contact email	richard.zimmermann@uks.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD011993

PRIDE project URI

• PRIDE
  1. PXD011993
     1. Label: PRIDE project
     2. Name: Targeting of precursor polypeptides to the human ER