PXD011987

PXD011987 is an original dataset announced via ProteomeXchange.

Title	Cyclin Specificity Governs Centrosomal Cyclin/CDK Localisation and is Necessary for the G2/M Transition
Description	In eukaryotes, cyclin/CDK complexes drive cells through DNA replication and mitosis, establising cell cycle temporal order. The fission yeast S. pombe is able to function with a single cyclin/CDK complex, with cell cycle order emerging from differential substrate sensitivities to CDK activity. What influence cyclin specificity has upon the cell cycle in this situation is currently unknown. Here we show that in a system that is reliant on a single cyclin for both the G1/S and G2/M transition, cyclin specificity is not needed for the G1/S transition, but instead is required for the G2/M transition and correct localisation of cyclin/CDK to the yeast centrosome equivalent, the SPB. This loss of centrosome localisation was also seen in human cells expressing mutant Cyclin B1, suggesting conservation of function. In fission yeast, although interphase centrosomal localisation is lost, mitotic SPB localisation remains. This hitherto unknown second localisation method is dependent on Polo kinase, suggesting a finer spatiotemporal control of cyclin/CDK during cell cycle progression than previously known. Thus, cyclin specificity is intrinsically twinned with spatial control of cyclin/CDK, and we suggest that this regulation may be conserved through evolution.
HostingRepository	PRIDE
AnnounceDate	2019-05-27
AnnouncementXML	Submission_2019-05-27_00:58:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Jones
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-10 01:55:09	ID requested
⏵ 1	2019-05-27 00:58:17	announced

Velez G, Yang J, Li AS, Tsang SH, Bassuk AG, Mahajan VB, Proteomic insight into the pathogenesis of CAPN5-vitreoretinopathy. Sci Rep, 9(1):7608(2019) [pubmed]

curator keyword: Biological

submitter keyword: CDK Mitosis TMT Phosphorylation

Bram Snijders
contact affiliation	Mass Spectrometry Proteomics Platform The Francis Crick Institute
contact email	Bram.Snijders@crick.ac.uk
lab head
Andrew Jones
contact affiliation	The Francis Crick Institute
contact email	Andrew.Jones@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011987
     1. Label: PRIDE project
     2. Name: Cyclin Specificity Governs Centrosomal Cyclin/CDK Localisation and is Necessary for the G2/M Transition