PXD011984 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | UHPLC-MS/MS analysis of the proteomic profiles of cocoa beans from different genotypes |
Description | Amongst other compounds, cocoa flavour is dependent on peptides that are formed during fermentation of cocoa beans from proteins such as albumin and vicilin. In this study the proteomic profiles of cocoa beans from four genotypes (ICS 1, ICS 39, IMC 67 and SCA 6) with different genetic background and flavour profiles have been analysed by employing a bottom-up label free LC-MS/MS approach. From a total of 430 identified proteins, 250 proteins were found significantly differentially expressed among the four cocoa genotypes analysed. Of these, 61 proteins with a fold change of 2 or more were further investigated, showing that the majority is involved in stress response. Furthermore, several of these 61 proteins could also be linked to oxidation-reduction processes. PCA analysis allowed a clear separation of the genotypes based on their proteomic profile, with an aminohydrolase and a sulphite oxidase greatly contributing to the separation. Aspartyl protease was more abundant in the genotypes ICS 1 and ICS compared to IMC 67, while a serine carboxypeptidase was significantly more expressed in the genotype ICS 39 in comparison with the other genotypes. Both these enzymes catalyse the degradation of storage proteins during fermentation. A Beta-amylase, an enzyme which catalyses the release of maltose was detected at a significantly higher level in the genotype SCA 6 compared to ICS 1 and IMC 67. Two Amine oxidases were significantly more abundant in the genotype SCA 6 compared to ICS 39, and in the genotype ICS 1 versus ICS 39, while two alcohol dehydrogenase were higher expressed in the genotype SCA 6 compared to IMC 67. These enzyme catalyse oxidation of amines and alcohols with release of aldehydes and ketones. The data shows that UHPLC-MS/MS can be employed to differentiate cocoa beans from various varieties, and thus in theory be linked to differences in their flavour profile. |
HostingRepository | PRIDE |
AnnounceDate | 2019-08-15 |
AnnouncementXML | Submission_2019-08-30_00:43:55.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD011984 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Emanuele Scollo |
SpeciesList | scientific name: Theobroma cacao (Cacao) (Cocoa); NCBI TaxID: 3641; |
ModificationList | Oxidation; Acetyl; Carbamidomethyl |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-10 01:42:37 | ID requested | |
1 | 2019-08-15 02:56:14 | announced | |
⏵ 2 | 2019-08-30 00:43:56 | announced | Updated publication reference for PubMed record(s): 31445177. |
Publication List
Scollo E, Neville DCA, Oruna-Concha MJ, Trotin M, Cramer R, UHPLC-MS/MS analysis of cocoa bean proteomes from four different genotypes. Food Chem, 303():125244(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Cocoa beans, label free quantitation, plant proteomics, cocoa beans proteome, Theobroma cacao |
Contact List
Professor Rainer Cramer |
contact affiliation | University of Reading |
contact email | r.k.cramer@rdg.ac.uk |
lab head | |
Emanuele Scollo |
contact affiliation | University of Reading |
contact email | e.scollo@hotmail.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011984
- Label: PRIDE project
- Name: UHPLC-MS/MS analysis of the proteomic profiles of cocoa beans from different genotypes