PXD011974

PXD011974 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling proteomics of the androgen receptor reveals Kruppel-Like Factor 4 as a repressor of androgen-dependent genes
Description	Prostate cancer (PCa) is the most frequently diagnosed cancer in Canadian men and is the third cause of cancer mortality. PCa initiation and growth is driven by the androgen receptor (AR). AR is activated by androgens such as testosterone and controls prostatic cell proliferation and survival. We sought to characterize global AR signalling networks. We performed BioID proximity labeling proteomics in androgen-dependent LAPC4 cells to delineate AR protein interaction networks. We report the identification of 32 AR associated proteins in non-stimulated cells. Strikingly, the AR signalling network increased to 183 and 201 proteins, upon 24h or 72h androgenic stimulation, respectively. Among this group, we identified 215 proteins that were not previously reported as AR interactors. Interestingly, these AR associated proteins were previously reported to be involved in DNA metabolism, RNA processing and RNA polymerase II transcription. Moreover, we identified 44 transcription factors, such as the Krüppel-like factor 4 (KLF4), which was specifically revealed in androgen-stimulated cells. We determined that KLF4 acts as a repressor of AR target genes transcription in PCa cells. Taken together, our data report the largest high-confidence proximity networks for AR in PCa cells.
HostingRepository	PRIDE
AnnounceDate	2021-03-02
AnnouncementXML	Submission_2021-03-01_22:18:18.084.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011974
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Velot
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Deamidated; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-07 01:17:24	ID requested
1	2021-03-01 08:21:59	announced
⏵ 2	2021-03-01 22:18:18	announced	2021-03-02: Updated project metadata.

10.1016/J.MCPRO.2021.100064;

V, é, lot L, Lessard F, B, é, rub, é, -Simard FA, Tav C, Neveu B, Teyssier V, Boudaoud I, Dionne U, Lavoie N, Bilodeau S, Pouliot F, Bisson N, Proximity-dependent Mapping of the Androgen Receptor Identifies Kruppel-like Factor 4 as a Functional Partner. Mol Cell Proteomics, 20():100064(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: AR, proximity, network, KLF4

Nicolas Bisson
contact affiliation	Centre de recherche du Centre Hospitalier Universitaire (CHU) de Québec-Université Laval, Axe Oncologie, Québec, QC, Canada
contact email	Nick.Bisson@crchudequebec.ulaval.ca
lab head
Lauriane Velot
contact affiliation	Centre de Recherche du CHU de Québec
contact email	lauriane.velot@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD011974

PRIDE project URI

• PRIDE
  1. PXD011974
     1. Label: PRIDE project
     2. Name: Proximity labeling proteomics of the androgen receptor reveals Kruppel-Like Factor 4 as a repressor of androgen-dependent genes