PXD011970

PXD011970 is an original dataset announced via ProteomeXchange.

Title	Post-column solvent addition enhances sensitivity of microflow-based bottom-up proteomics
Description	We demonstrate that post-column addition of organic solvents leads to a strong enhancement of electrospray stability in microflow LC-MS experiments improving the sensitivity across the entire gradient, for early eluting peptides by up to tenfold. Post-column solvent addition did not negatively affect chromatographic performance and resulted in an overall 28%-36% increase in identifications at both protein- and peptide-level. Post-column solvent addition can be easily adopted on any microflow LC-MS/MS system.
HostingRepository	PRIDE
AnnounceDate	2019-05-28
AnnouncementXML	Submission_2019-05-28_01:06:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mateusz Lacki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-06 23:44:49	ID requested
⏵ 1	2019-05-28 01:06:38	announced

Distler U, Ł, ą, cki MK, Schumann S, Wanninger M, Tenzer S, Enhancing Sensitivity of Microflow-Based Bottom-Up Proteomics through Postcolumn Solvent Addition. Anal Chem, 91(12):7510-7515(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Bottom-up proteomics, Microflow LC-MS, Post-column solvent addition, HeLa digest

Stefan Tenzer
contact affiliation	University Medical Center of the Johannes Gutenberg University Mainz
contact email	tenzer@uni-mainz.de
lab head
Mateusz Lacki
contact affiliation	University Medical Center
contact email	matteo.lacki@uni-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD011970

PRIDE project URI

• PRIDE
  1. PXD011970
     1. Label: PRIDE project
     2. Name: Post-column solvent addition enhances sensitivity of microflow-based bottom-up proteomics