PXD011968

PXD011968 is an original dataset announced via ProteomeXchange.

Title	Stk38 interactomics by proximity ligation
Description	STK38 (aka NDR1) is a Hippo pathway serine/threonine protein kinase with multifarious functions in normal and cancer cells. Using a context-dependent proximity labelling assay, we discovered that STK38 modulates its interaction with more than 250 proteins depending on the context. Upon starvation-induced autophagy, STK38 associates with cytoplasmic proteins while upon ECM detachment it binds to mainly nuclear proteins. This differential subcellular-localization-dependent activity is caused by the XPO1 (Exportin-1, CRM1) dependent nuclear/cytoplasmic shuttling of STK38. STK38 associates with nuclear related partners upon ECM detachment and with cytoplasmic related ones upon autophagy, exhibiting a nuclear/cytoplasmic shuttling under the dependency of XPO1 (Exportin-1, aka CRM1). We further uncovered that the XPO1-mediated export of STK38 is dependent on phosphorylation of XPO1s serin residue 1055 by STK38 itself. In addition to regulating its own nuclear export, STK38 also controls the subcellular distribution of Beclin1, a key regulator of autophagy. Moreover, the regulation of XPO1 by STK38 mediates the nuclear exclusion of YAP1. Collectively, our results reveal that functions of STK38 are linked to the XPO1-mediated subcellular distribution of STK38 and key regulators. These observations show that unrelated cellular functions can be regulated by a same mechanism controlled by a single kinase and demonstrate a novel mechanism of XPO1-dependent cargo export regulation y phosphorylation of XPO1s C-terminal auto-inhibitory domain.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:51:20.069.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chiara guerrera
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-06 07:50:24	ID requested
1	2019-09-25 19:54:44	announced
2	2019-09-30 00:49:50	announced	2019-09-30: Updated publication reference for PubMed record(s): 31544310.
⏵ 3	2024-10-22 04:51:20	announced	2024-10-22: Updated project metadata.

Martin AP, Jacquemyn M, Lipecka J, Chhuon C, Aushev VN, Meunier B, Singh MK, Carpi N, Piel M, Codogno P, Hergovich A, Parrini MC, Zalcman G, Guerrera IC, Daelemans D, Camonis JH, STK38 kinase acts as XPO1 gatekeeper regulating the nuclear export of autophagy proteins and other cargoes. EMBO Rep, 20(11):e48150(2019) [pubmed]

10.15252/embr.201948150;

submitter keyword: XPO1, STK38,APEX

I Chiara GUERRERA
contact affiliation	Proteomic platform 3P5-Necker, Université Paris Descartes, SFR Necker, INSERM US24/ CNRS UMS3633 Paris
contact email	chiara.guerrera@inserm.fr
lab head
Chiara guerrera
contact affiliation	Necker proteomics, INSERM
contact email	chiara.guerrera@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD011968

PRIDE project URI

• PRIDE
  1. PXD011968
     1. Label: PRIDE project
     2. Name: Stk38 interactomics by proximity ligation