PXD011963 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The SUMO protease SENP6 regulates the constitutive centromere-associated network by group |
Description | Project 1: Identification of SUMOylated proteins which are regulated by the SUMO protease SENP6. SENP6 knockdown was performed by two independent shRNAs and SUMOylated proteins were purified from U2OS expressing His10-tagged SUMO2 by means of Ni-NTA pulldown. Purified SUMOylated proteins were identified and quantified by label-free quantification. Project 2: Dynamics of chromatin-associated proteins influenced by SENP6. After siRNA-mediated knockdown of SENP6, chromatin fractions of U2OS cells were isolated and abundances of proteins were quantified. Project 3: Analysis of in vitro SUMOylated CENP-T for the presence of SUMOylated SUMO peptides. For this aim purified CENP-T-HA was in vitro SUMOylated with a SUMO Q87R mutant to reduce the length of the tryptic peptide and therefore to be able to identified sit-specific SUMOylation. |
HostingRepository | PRIDE |
AnnounceDate | 2019-09-13 |
AnnouncementXML | Submission_2019-09-13_02:37:54.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Frauke Liebelt |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-06 06:08:16 | ID requested | |
⏵ 1 | 2019-09-13 02:37:55 | announced | |
Publication List
Keyword List
curator keyword: Biological |
submitter keyword: human osteosacrcoma cell line U2OS, SENP6, Chromatin |
Contact List
Alfred C.O. Vertegaal |
contact affiliation | Chemical Cell Biology Leiden Univerity Medical Center Einthovenweg 20 2333ZC Leiden, The Netherlands |
contact email | a.c.o.vertegaal@lumc.nl |
lab head | |
Frauke Liebelt |
contact affiliation | Leiden University Medical Centre |
contact email | F.Liebelt@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD011963 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011963
- Label: PRIDE project
- Name: The SUMO protease SENP6 regulates the constitutive centromere-associated network by group