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PXD011963

PXD011963 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe SUMO protease SENP6 regulates the constitutive centromere-associated network by group
DescriptionProject 1: Identification of SUMOylated proteins which are regulated by the SUMO protease SENP6. SENP6 knockdown was performed by two independent shRNAs and SUMOylated proteins were purified from U2OS expressing His10-tagged SUMO2 by means of Ni-NTA pulldown. Purified SUMOylated proteins were identified and quantified by label-free quantification. Project 2: Dynamics of chromatin-associated proteins influenced by SENP6. After siRNA-mediated knockdown of SENP6, chromatin fractions of U2OS cells were isolated and abundances of proteins were quantified. Project 3: Analysis of in vitro SUMOylated CENP-T for the presence of SUMOylated SUMO peptides. For this aim purified CENP-T-HA was in vitro SUMOylated with a SUMO Q87R mutant to reduce the length of the tryptic peptide and therefore to be able to identified sit-specific SUMOylation.
HostingRepositoryPRIDE
AnnounceDate2019-09-13
AnnouncementXMLSubmission_2019-09-13_02:37:54.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrauke Liebelt
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-06 06:08:16ID requested
12019-09-13 02:37:55announced
Publication List
Keyword List
curator keyword: Biological
submitter keyword: human osteosacrcoma cell line U2OS, SENP6, Chromatin
Contact List
Alfred C.O. Vertegaal
contact affiliationChemical Cell Biology Leiden Univerity Medical Center Einthovenweg 20 2333ZC Leiden, The Netherlands
contact emaila.c.o.vertegaal@lumc.nl
lab head
Frauke Liebelt
contact affiliationLeiden University Medical Centre
contact emailF.Liebelt@lumc.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
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