PXD011956

PXD011956 is an original dataset announced via ProteomeXchange.

Title	Norovirus infection results in assembly of virus-specific G3BP1 granules and evasion of eIF2 apha signaling
Description	Proteomic analysis of murine stress granule and G3BP1 granulome in infected cells. BV2 GFP-G3BP1 cells were either treated with 0.1 mM arsenite for 1h to induce SG assembly or infected with MNV for 9h. G3BP1 granules were enriched by sequential centrifugation and purified by immunoprecipitation using antibodies to GFP (to trap GFP-G3BP1) or IgG (as a control) followed by pull down with Protein A-conjugated epoxy Dynabeads as previously described. To characterize the identity of G3BP1 partners within these, Mass Spectrometric analysis was performed.
HostingRepository	PRIDE
AnnounceDate	2019-12-12
AnnouncementXML	Submission_2020-01-24_00:12:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valentina Iadevaia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-06 03:15:10	ID requested
1	2019-12-12 04:03:19	announced
⏵ 2	2020-01-24 00:12:06	announced	2020-01-24: Updated publication reference for PubMed record(s): 31905230.

Brocard M, Iadevaia V, Klein P, Hall B, Lewis G, Lu J, Burke J, Willcocks MM, Parker R, Goodfellow IG, Ruggieri A, Locker N, independent host translation shut-off and remodels the G3BP1 interactome evading stress granule formation. PLoS Pathog, 16(1):e1008250(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: G3BP1, MNV, stress granules, arsenite

Nicolas Locker
contact affiliation	University of Surrey
contact email	N.Locker@surrey.ac.uk
lab head
Valentina Iadevaia
contact affiliation	University of Surrey
contact email	v.iadevaia@surrey.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD011956

PRIDE project URI

• PRIDE
  1. PXD011956
     1. Label: PRIDE project
     2. Name: Norovirus infection results in assembly of virus-specific G3BP1 granules and evasion of eIF2 apha signaling