PXD011948

PXD011948 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS after anti-PUM1 and anti-PUM2 co-immunoprecipitation.
Description	In order to identify the protein co-factors of PUMILIO1 and PUMILIO2 in TCam-2 cell line, co-IP of these proteins performed followed mass spectrometry analysis for protein identification. Six biological replicates of co-IP with anti-PUM1, anti-PUM2 antibodies and anti-IgG were performed. Three biological replicates were performed without RNase A treatment, and another three biological replicates of co-IPs with 100 mg/ml RNase A.
HostingRepository	PRIDE
AnnounceDate	2020-04-27
AnnouncementXML	Submission_2020-04-26_23:11:56.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011948
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Erkut Ilaslan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Ammonia-loss; Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-06 01:23:45	ID requested
⏵ 1	2020-04-26 23:11:58	announced

Smialek MJ, Ilaslan E, Sajek MP, Swiercz A, Janecki DM, Kusz-Zamelczyk K, Wozniak T, Kotecki M, Handschuh L, Figlerowicz M, Jaruzelska J, Characterization of RNP Networks of PUM1 and PUM2 Post-Transcriptional Regulators in TCam-2 Cells, a Human Male Germ Cell Model. Cells, 9(4):(2020) [pubmed]

curator keyword: Biological

submitter keyword: Human, TCam-2, Co-IP, pumilio

Jadwiga Jaruzelska
contact affiliation	Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
contact email	jadwiga.jaruzelska@igcz.poznan.pl
lab head
Erkut Ilaslan
contact affiliation	Polish Academy of Sciences
contact email	erkutilaslan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD011948

PRIDE project URI

• PRIDE
  1. PXD011948
     1. Label: PRIDE project
     2. Name: LC-MS/MS after anti-PUM1 and anti-PUM2 co-immunoprecipitation.