PXD011946

PXD011946 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	BioID2_PHB2(V1)-(WT_KKK_I225P)
Description	HEK293 cells that stably express wild-type (WT), E229/231/233K (KKK), or I225P mutant PHB2(V1) fused at C-terminus with the biotin ligase from A. aeolicus were incubated with 100 μM biotin for 18 h. The mitochondrial fractions were lysed in RIPA buffer, and the clarified supernatants were incubated with streptavidin-agarose beads for 2 h at 4° C. Proteins on the beads were digested by adding trypsin/Lys-C mix for 16 h at 37° C and analyzed by LC-MS/MS. Label-free quantification was performed using Proteome Discoverer 2.2 software.
HostingRepository	jPOST
AnnounceDate	2019-09-05
AnnouncementXML	Submission_2022-09-18_02:29:47.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hidetaka Kosako
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; alpha-amino acetylated residue; unknown modification
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-05 19:53:25	ID requested
1	2019-09-05 00:48:45	announced
2	2019-09-05 05:34:21	announced	2019-09-05: Updated title.
3	2019-09-26 00:03:09	announced	2019-09-26: Updated PubMed.
4	2021-02-04 17:21:33	announced	2021-02-04: Updated FTP location.
⏵ 5	2022-09-18 02:29:48	announced	2022-09-18: Updated FTP location.

Publication List

Yoshinaka T, Kosako H, Yoshizumi T, Furukawa R, Hirano Y, Kuge O, Tamada T, Koshiba T, Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region. iScience, 19():1065-1078(2019) [pubmed]

Yoshinaka T, Kosako H, Yoshizumi T, Furukawa R, Hirano Y, Kuge O, Tamada T, Koshiba T, Structural Basis of Mitochondrial Scaffolds by Prohibitin Complexes: Insight into a Role of the Coiled-Coil Region. iScience, 19():1065-1078(2019) [pubmed]

Keyword List

submitter keyword: BioID

submitter keyword: BioID

Contact List

Hidetaka Kosako
lab head
Hidetaka Kosako
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000529/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000529/