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PXD011945

PXD011945 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleXcc Pan Proteome
DescriptionIn this project, two isolates of Xanthomonas campestris pv campestris were compared in the following conditions, 1. growth in NYG rich medium, and 2. growth in XVM1 minimal medium. Total proteins were extracted and trypsin digested. The nanoscale Liquid Chromatography was performed to separate tryptic peptides in a nanoAcquity system (Waters, USA) using 2D dilution technology. The digested peptides were analyzed in a Synapt G2 HDMS mass spectrometer (Waters, Manchester, UK) operated in the resolution mode of analysis and a resolving power of at least 20,000 full-width half-maximum (FWHM). All analyses were performed using a positive nanoelectrospray ion mode (nanoESI +). ProteinLynx Global Server (PLGS) version 2.5 (Waters, Manchester, UK) was used to process the MS data obtained from LC-MSE. For protein identification, the software embedded ion accounting algorithm was used. For the analysis of protein identification and quantification level, the observed intensity measurements were auto normalized by PLGS.
HostingRepositoryMassIVE
AnnounceDate2018-12-07
AnnouncementXMLSubmission_2018-12-07_03:36:11.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFabiano Touzdjian
SpeciesList scientific name: Xanthomonas campestris pv. campestris; NCBI TaxID: 340;
ModificationListCarbamyl; Oxidation
InstrumentSynapt G2 HDMS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-05 11:16:26ID requested
12018-12-07 03:36:12announced
Publication List
no publication
Keyword List
submitter keyword: Shotgun proteomics, Xcc, Pan Proteome,
Contact List
Angela Mehta
contact affiliationEmbrapa
contact emailangela.mehta@embrapa.br
lab head
Fabiano Touzdjian
contact affiliationembrapa
contact emailfabianotouz@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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