PXD011938

PXD011938 is an original dataset announced via ProteomeXchange.

Title	2D gel-based proteomics of primary human peritoneal mesothelial cells derived from omentum
Description	Peritoneal mesothelial cells are harmed by peritoneal dialysis fluids (PDF) used in renal replacement therapy with peritoneal dialysis. The mechanisms of the cellular damage are not yet described in detail. Primary human peritoneal mesothelial cells derived from omentum of five donors were independently exposed to peritoneal dialysis fluids. The extent of cell damage was assessed using lactate dehydrogenase (LDH) release in the cell culture supernatant and cells were lysed in order to extract mRNA and proteins. Transcriptional changes induced by PDF were analyzed using gene expression microarrays and changes of the proteome were analyzed using 2D-electrophoresis.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:05:25.939.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011938
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Klaus Kratochwill
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	MALDI LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-05 02:44:32	ID requested
⏵ 1	2022-02-22 04:05:26	announced

Herzog R, Sacnun JM, Gonz, á, lez-Mateo G, Bartosova M, Bialas K, Wagner A, Unterwurzacher M, Sobieszek IJ, Daniel-Fischer L, Rusai K, Pascual-Ant, ó, n L, Kaczirek K, Vychytil A, Schmitt CP, L, ó, pez-Cabrera M, Alper SL, Aufricht C, Kratochwill K, B-crystallin. Sci Transl Med, 13(608):(2021) [pubmed]

submitter keyword: Human, 2D-DIGE, Peritoneal Dialysis, MALDI

Dr Klaus Kratochwill
contact affiliation	Medical University of Vienna
contact email	klaus.kratochwill@meduniwien.ac.at
lab head
Klaus Kratochwill
contact affiliation	Medical University of Vienna
contact email	klaus.kratochwill@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD011938

PRIDE project URI

• PRIDE
  1. PXD011938
     1. Label: PRIDE project
     2. Name: 2D gel-based proteomics of primary human peritoneal mesothelial cells derived from omentum