PXD011922

PXD011922 is an original dataset announced via ProteomeXchange.

Title	Proteomics of testis from SPPL2c KO mice
Description	Signal peptide peptidase (SPP) and the four homologous SPP-like (SPPL) proteases constitute a family of intramembrane aspartyl proteases with selectivity for type II-oriented transmembrane segments. Here, we have analysed the physiological function of the orphan protease SPPL2c, previously considered to represent a non-expressed pseudogene. We identified proteolytic activity of SPPL2c towards selected tail-anchored proteins. Despite shared ER localization, SPPL2c and SPP exhibit distinct, though partially overlapping substrate spectra and inhibitory profiles and are organised in different high molecular-weight complexes. Unexpectedly, SPPL2c is specifically expressed in murine and human testis where it is primarily localised in spermatids. In mice, SPPL2c-deficiency leads to a loss of elongated spermatids and reduced motility of mature spermatozoa, but preserved fertility. Additionally, matings of male and female SPPL2c-/- mice exhibit reduced litter sizes. Using proteomics we identified the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA2)-regulating protein phospholamban (PLN) as a physiological SPPL2c substrate. Accumulation of PLN correlates with a decrease of intracellular Ca2+ levels in elongated spermatids that likely contributes to the compromised male germ cell differentiation and function of SPPL2c-/- mice.
HostingRepository	PRIDE
AnnounceDate	2019-02-15
AnnouncementXML	Submission_2019-02-15_04:00:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-03 05:19:01	ID requested
⏵ 1	2019-02-15 04:00:05	announced

Niemeyer J, Mentrup T, Heidasch R, M, ü, ller SA, Biswas U, Meyer R, Papadopoulou AA, Dederer V, Haug-Kr, ö, per M, Adamski V, L, ü, llmann-Rauch R, Bergmann M, Mayerhofer A, Saftig P, Wennemuth G, Jessberger R, Fluhrer R, Lichtenthaler SF, Lemberg MK, Schr, ö, der B, phospholamban. EMBO Rep, 20(3):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Mouse, Testis

Bernd Schröder
contact affiliation	Institute for Physiological Chemistry Medizinisch-Theoretisches Zentrum MTZ Technical University Dresden Fiedlerstraße 42 D-01307 Dresden
contact email	bernd.schroeder@tu-dresden.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011922
     1. Label: PRIDE project
     2. Name: Proteomics of testis from SPPL2c KO mice