PXD011914 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Interactions of human norovirus capsid P domain with histo-blood group antigens with Hydrogen-Deuterium Exchange Mass Spectrometry |
Description | Attachment of human noroviruses to histo blood group antigens (HBGAs) is essential for infection, but how this binding event promotes the infection of host cells is unknown. Here, we employed protein NMR experiments supported by mass spectrometry and crystallography to study HBGA binding to the P domain of a prevalent virus strain (GII.4) in more detail . We report a highly selective transformation of N373, located in an antigenic loop adjoining the HBGA binding site, into an iso-aspartate residue. This spontaneous post-translational modification (PTM) proceeds with an estimated half-life of a few days at physiological temperatures, independent of the presence of HBGAs but dramatically affecting HBGA recognition. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:51:20.279.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jasmin Dülfer |
SpeciesList | scientific name: Norovirus Hu/GII-4/Saga4/2006/JP; NCBI TaxID: 546981; scientific name: Norovirus Hu/GII.4/MI001/2011/USA; NCBI TaxID: 1354724; scientific name: Norovirus Hu/GII/JP/2015/GII.P17_GII.17/Kawasaki308; NCBI TaxID: 1634342; scientific name: Norwalk virus; NCBI TaxID: 11983; |
ModificationList | monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Fusion ETD |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-12-03 01:42:11 | ID requested | |
1 | 2019-03-20 06:27:47 | announced | |
2 | 2019-04-03 04:36:01 | announced | Updated publication reference for PubMed record(s): 30899001. |
⏵ 3 | 2024-10-22 04:51:28 | announced | 2024-10-22: Updated project metadata. |
Publication List
Mallagaray A, Creutznacher R, D, ü, lfer J, Mayer PHO, Grimm LL, Ordu, ñ, a JM, Trabjerg E, Stehle T, Rand KD, Blaum BS, Uetrecht C, Peters T, A post-translational modification of human Norovirus capsid protein attenuates glycan binding. Nat Commun, 10(1):1320(2019) [pubmed] |
10.1038/s41467-019-09251-5; |
Keyword List
curator keyword: Biomedical |
submitter keyword: HDX MS, P domain, GII.4 Saga,human norovirus, histo-blood group antigens |
Contact List
Charlotte Uetrecht |
contact affiliation | Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, 20251 Hamburg, Germany |
contact email | charlotte.uetrecht@xfel.eu |
lab head | |
Jasmin Dülfer |
contact affiliation | Heinrich Pette Institute |
contact email | jasmin.duelfer@leibniz-hpi.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011914
- Label: PRIDE project
- Name: Interactions of human norovirus capsid P domain with histo-blood group antigens with Hydrogen-Deuterium Exchange Mass Spectrometry