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PXD011914

PXD011914 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInteractions of human norovirus capsid P domain with histo-blood group antigens with Hydrogen-Deuterium Exchange Mass Spectrometry
DescriptionAttachment of human noroviruses to histo blood group antigens (HBGAs) is essential for infection, but how this binding event promotes the infection of host cells is unknown. Here, we employed protein NMR experiments supported by mass spectrometry and crystallography to study HBGA binding to the P domain of a prevalent virus strain (GII.4) in more detail . We report a highly selective transformation of N373, located in an antigenic loop adjoining the HBGA binding site, into an iso-aspartate residue. This spontaneous post-translational modification (PTM) proceeds with an estimated half-life of a few days at physiological temperatures, independent of the presence of HBGAs but dramatically affecting HBGA recognition.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:51:20.279.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJasmin Dülfer
SpeciesList scientific name: Norovirus Hu/GII-4/Saga4/2006/JP; NCBI TaxID: 546981; scientific name: Norovirus Hu/GII.4/MI001/2011/USA; NCBI TaxID: 1354724; scientific name: Norovirus Hu/GII/JP/2015/GII.P17_GII.17/Kawasaki308; NCBI TaxID: 1634342; scientific name: Norwalk virus; NCBI TaxID: 11983;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-12-03 01:42:11ID requested
12019-03-20 06:27:47announced
22019-04-03 04:36:01announcedUpdated publication reference for PubMed record(s): 30899001.
32024-10-22 04:51:28announced2024-10-22: Updated project metadata.
Publication List
Mallagaray A, Creutznacher R, D, ü, lfer J, Mayer PHO, Grimm LL, Ordu, ñ, a JM, Trabjerg E, Stehle T, Rand KD, Blaum BS, Uetrecht C, Peters T, A post-translational modification of human Norovirus capsid protein attenuates glycan binding. Nat Commun, 10(1):1320(2019) [pubmed]
10.1038/s41467-019-09251-5;
Keyword List
curator keyword: Biomedical
submitter keyword: HDX MS, P domain, GII.4 Saga,human norovirus, histo-blood group antigens
Contact List
Charlotte Uetrecht
contact affiliationHeinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, 20251 Hamburg, Germany
contact emailcharlotte.uetrecht@xfel.eu
lab head
Jasmin Dülfer
contact affiliationHeinrich Pette Institute
contact emailjasmin.duelfer@leibniz-hpi.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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