PXD011913

PXD011913 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	E.coli and Human proteome
Description	To measure quantification accuracy, we generated two proteome samples of E.coli and human cell lysate. E.coli tryptic peptides were tagged with four different labels of reporter ions (m/z: 114, 115, 116 and 117). These labeled peptides were mixed at the following ratios: 114:115 = 10:1, 114:117 = 5:1, 114:116 and 117:115 = 2:1, and 116:117 = 2.5:1. Human tryptic peptides were tagged with two different labels of reporter ions at 114 and 115, and these samples were mixed 1:1.
HostingRepository	jPOST
AnnounceDate	2019-05-14
AnnouncementXML	Submission_2022-09-18_02:18:11.825.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iwasaki Mio
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); alpha-amino acetylated residue; Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-12-02 00:05:49	ID requested
1	2019-05-14 02:17:04	announced
2	2021-02-04 16:54:54	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 02:18:12	announced	2022-09-18: Updated FTP location.

Publication List

Iwasaki M, Tabata T, Kawahara Y, Ishihama Y, Nakagawa M, Removal of Interference MS/MS Spectra for Accurate Quantification in Isobaric Tag-Based Proteomics. J Proteome Res, 18(6):2535-2544(2019) [pubmed]

Iwasaki M, Tabata T, Kawahara Y, Ishihama Y, Nakagawa M, Removal of Interference MS/MS Spectra for Accurate Quantification in Isobaric Tag-Based Proteomics. J Proteome Res, 18(6):2535-2544(2019) [pubmed]

Keyword List

submitter keyword: iTRAQ, whole proteome

submitter keyword: iTRAQ, whole proteome

Contact List

Mio Iwasaki
lab head
Iwasaki Mio
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000492/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000492/