PXD011904

PXD011904 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	PU.1 interactome mouse bone marrow derived dendritic cells
Description	Mouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected at day 10. 2x10E8 cells were used to prepare whole cell extracts and to perform PU.1 immunoprecipitaion with PU.1 antibody (T-21 Santa Cruz). IgG was used as control.
HostingRepository	MassIVE
AnnounceDate	2020-11-19
AnnouncementXML	Submission_2020-11-19_02:51:22.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Guti�rrez
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Oxidation; Acetyl
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-30 05:21:20	ID requested
⏵ 1	2020-11-19 02:51:22	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: PU.1, dendritic cells, mouse, bone marrow derived, LPS

submitter keyword: PU.1, dendritic cells, mouse, bone marrow derived, LPS

Contact List

Laura Gutierrez
contact affiliation	ISPA - University of Oviedo
contact email	gutierrezglaura@uniovi.es
lab head
Laura Guti�rrez
contact affiliation	ISPA - University of Oviedo
contact email	gutierrezglaura@uniovi.es
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083176/