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PXD011904

PXD011904 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePU.1 interactome mouse bone marrow derived dendritic cells
DescriptionMouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected at day 10. 2x10E8 cells were used to prepare whole cell extracts and to perform PU.1 immunoprecipitaion with PU.1 antibody (T-21 Santa Cruz). IgG was used as control.
HostingRepositoryMassIVE
AnnounceDate2020-11-19
AnnouncementXMLSubmission_2020-11-19_02:51:22.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Guti�rrez
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListOxidation; Acetyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-30 05:21:20ID requested
12020-11-19 02:51:22announced
Publication List
no publication
Keyword List
submitter keyword: PU.1, dendritic cells, mouse, bone marrow derived, LPS
Contact List
Laura Gutierrez
contact affiliationISPA - University of Oviedo
contact emailgutierrezglaura@uniovi.es
lab head
Laura Guti�rrez
contact affiliationISPA - University of Oviedo
contact emailgutierrezglaura@uniovi.es
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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