PXD011901

PXD011901 is an original dataset announced via ProteomeXchange.

Title	Hsa-miR-139-5p expression regulates the abundance of proteins related to poor survival cancer in CAL-62 thyroid cancer cell line
Description	We studied the impact of hsa-miR-139-5p on the protein output by means of an iTRAQ-based approach. First, we established two CAL-62 isogenic cell lines expressing either the mature hsa-miR-139-5p or a non-targeting control upon a doxycycline inducible promoter (PTRE3G-tGFP, Dharmacon). Total proteins of P-tGFP-hsa-miR139-5p untreated or treated with doxycycline (1ug/ml) for 96 and 120 hours were isolated and labeled with iTRAQ® reagent 8-plex. Two independent experiments were performed.
HostingRepository	PRIDE
AnnounceDate	2019-08-13
AnnouncementXML	Submission_2019-08-19_04:32:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduardo Zarzuela
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	impact

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-30 05:01:24	ID requested
1	2019-08-13 00:11:14	announced
⏵ 2	2019-08-19 04:32:56	announced	Updated publication reference for PubMed record(s): 31403184.

Montero-Conde C, Gra, ñ, a-Castro O, Mart, í, n-Serrano G, Mart, í, nez-Montes Á M, Zarzuela E, Mu, ñ, oz J, Torres-Perez R, Pita G, Cordero-Barreal A, Leandro-Garc, í, a LJ, Let, ó, n R, L, ó, pez de Silanes I, Guadalix S, P, é, rez-Barrios A, Hawkins F, Guerrero-, Á, lvarez A, Á, lvarez-Escol, á C, Regojo-Zapata RM, Calsina B, Remacha L, Rold, á, n-Romero JM, Santos M, Lanillos J, Jord, á M, Riesco-Eizaguirre G, Zafon C, Gonz, á, lez-Neira A, Blasco MA, Al-Shahrour F, Rodr, í, guez-Antona C, Casc, ó, n A, Robledo M, Hsa-miR-139-5p is a prognostic thyroid cancer marker involved in HNRNPF-mediated alternative splicing. Int J Cancer, 146(2):521-530(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Hsa-miR-139-5p, iTRAQ, Impact, CAL-62, thyroid cancer

Mercedes Robledo
contact affiliation	CNIO
contact email	mrobledo@cnio.es
lab head
Eduardo Zarzuela
contact affiliation	Centro Nacional de Investigaciones Oncológicas
contact email	eduzarfer@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD011901

PRIDE project URI

• PRIDE
  1. PXD011901
     1. Label: PRIDE project
     2. Name: Hsa-miR-139-5p expression regulates the abundance of proteins related to poor survival cancer in CAL-62 thyroid cancer cell line