PXD011890

PXD011890 is an original dataset announced via ProteomeXchange.

Title	Protein phosphorylation landscape of mouse spermatids during spermiogenesis
Description	Tadpole-shaped sperm is formed from round spermatid by spermiogenesis, a process with dramatic morphological changes in the final stage of spermatogenesis in testis. Protein phosphorylation, as one of the most important post-translational modifications, can regulate spermiogenesis, however, there is a lack of systemic analysis for phosphorylation events in this process. By large-scale phosphoproteome profiling using IMAC and TiO2 enrichment, we identified 18,980 phosphorylation sites in 4,628 phosphoproteins in mouse purified spermatids undergoing spermiogenesis. The identified phosphoproteins were significantly enriched in RNA transport, cell-cell adhesion, centrosome, microtubule and cilliogenesis. Further characterization of the kinase substrate relationship network demonstrated enrichment of phosphorylation substrates were related to the regulation of spermiogenesis. This global protein phosphorylation landscape of spermiogenesis showed wide phosphoregulation of diverse processes during spermiogenesis and could help further characterization of the process of sperm generation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:51:14.398.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	hao zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-29 07:46:28	ID requested
1	2019-03-29 06:39:51	announced
⏵ 2	2024-10-22 04:51:15	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201900055;

Li Y, Cheng Y, Zhu T, Zhang H, Li W, Guo Y, Qi Y, Chen X, Zhang J, Sha J, Zhou Z, Zhu H, Guo X, The Protein Phosphorylation Landscape of Mouse Spermatids during Spermiogenesis. Proteomics, 19(11):e1900055(2019) [pubmed]

submitter keyword: mouse spermatids,spermiogenesis,phosphorylation

Guo, Xuejiang
contact affiliation	Nanjing Med Univ, State Key Lab Reprod Med, Dept Histol & Embryol, 140 Hanzhong Rd, Nanjing 210029, Jiangsu, Peoples R China.
contact email	guo_xuejiang@njmu.edu.cn
lab head
hao zhang
contact affiliation	Nanjing medical university
contact email	542370159@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011890

PRIDE project URI

• PRIDE
  1. PXD011890
     1. Label: PRIDE project
     2. Name: Protein phosphorylation landscape of mouse spermatids during spermiogenesis