PXD011886

PXD011886 is an original dataset announced via ProteomeXchange.

Title	Biochemical and Molecular characterization of Super oxide dismutase enzyme purified from Ajwain (Trachyspermum ammi)
Description	Superoxide dismutase (EC 1.15.1.1) (SOD) is a standout amongst the most critical metal containing enzymes that can act as a main line of defense against oxidative stress. There has always been an increased interest globally to identify new plant sources with high SOD activity that are pharmacologically potent and have minimum side effects for their use as preventive medicine and in food industry. Trachyspermum ammi showed high SOD activity and the enzyme was purified to homogeneity using various downstream processing techniques like; Salt precipitation, dialysis, IEX, Gel permeation, NATIVE- and SDS-PAGE. Biochemical characterization with respect to optimum operational parameters viz., pH, Temperature, Stability, Concentration etc., was done. Kinetic studies for determination of Km, Vmax, Temperature coefficient, Activation energy have also been accomplished.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_02:45:32.307.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KANAGARETHINAM S
SpeciesList	scientific name: Viridiplantae (Green Plants; NCBI TaxID: NCBInr 20130519;
ModificationList	No PTMs are included in the dataset
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-29 03:56:16	ID requested
⏵ 1	2021-11-03 02:45:32	announced

Dataset with its publication pending

submitter keyword: Superoxide dismutase, Ajwain, Trachyspermum ammi, Molecular analysis, Biochemical characterization

Nidhee chaudhary
contact affiliation	Center for Biotechnology & Biochemical Engineering Amity Institute of Biotechnology Amity University Uttar Pradesh Sector-125, Noida-201313 (U.P.) India
contact email	nchaudhary@amity.edu
lab head
KANAGARETHINAM S
contact affiliation	PhD scholar
contact email	rethinam23@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD011886

PRIDE project URI

• PRIDE
  1. PXD011886
     1. Label: PRIDE project
     2. Name: Biochemical and Molecular characterization of Super oxide dismutase enzyme purified from Ajwain (Trachyspermum ammi)