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PXD011879

PXD011879 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMS-based identification of AcP10 interaction partners.
DescriptionIn times of cellular stress, such as during virus infections, the integrated stress response (ISR) blocks translation initiation through phosphorylation of the essential translation initiation factor eIF2. Phosphorylated eIF2 (p-eIF2) sequesters the eIF2-specific guanidine exchange factor (GEF) eIF2B, thereby preventing eIF2 recycling. Here we describe the first example of a viral ISR antagonist that inhibits the ISR at its most central step: the interplay between p-eIF2 and eIF2B. Using AP-MS, we determine that BW10 binds eIF2B. There, it selectively displaces eIF2B’s inhibitor p-eIF2 without affecting the association of its substrate eIF2. By this mechanism, BW10 renders cellular translation immune to regulation by eIF2 phosphorylation. Thus, under stress conditions BW10 creates the unprecedented situation of high levels of p-eIF2 coinciding with unimpaired translation.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:22.961.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPiero Giansanti
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-29 01:31:35ID requested
12020-07-24 03:38:38announced
22023-11-14 08:51:23announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Biological
submitter keyword: AP-MS, eIF2,BW10, interactome, eIF2B
Contact List
Albert J. R. Heck
contact affiliationBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact emailA.J.R.Heck@uu.nl
lab head
Piero Giansanti
contact affiliationBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact emailp.giansanti.uu@gmail.com
dataset submitter
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Dataset FTP location
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