PXD011877

PXD011877 is an original dataset announced via ProteomeXchange.

Title	Protein aggregation mediates protein complex stoichiometry in aneuploid cells, part 4
Description	Aneuploidy, a condition characterized by chromosome gains and losses, causes reduced fitness and numerous cellular stresses, including increased protein aggregation. Here, we identify protein complex stoichiometry imbalances as a major cause of protein aggregation in aneuploid cells. Subunits of protein complexes encoded on excess chromosomes aggregate in aneuploid cells, which is suppressed when expression of other subunits is coordinately altered. We further show that excess subunits are either degraded or aggregate and that protein aggregation is nearly as effective as protein degradation at lowering levels of excess proteins. Our study explains why proteotoxic stress is a universal feature of the aneuploid state and reveals protein aggregation as a form of dosage compensation to cope with disproportionate expression of protein complex subunits.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_12:40:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Vaites
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	6x(13)C labeled residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-29 01:24:21	ID requested
⏵ 1	2020-05-26 12:40:28	announced

Brennan CM, Vaites LP, Wells JN, Santaguida S, Paulo JA, Storchova Z, Harper JW, Marsh JA, Amon A, Protein aggregation mediates stoichiometry of protein complexes in aneuploid cells. Genes Dev, 33(15-16):1031-1047(2019) [pubmed]

curator keyword: Biological

submitter keyword: aggregate, aneuploidy, yeast, proteostasis

Angelika Amon
contact affiliation	David H. Koch Institute for Integrative Cancer Research Howard Hughes Medical Institute Massachusetts Institute of Technology Cambridge, MA 02139, USA
contact email	angelika@mit.edu
lab head
Laura Vaites
contact affiliation	Harvard Medical School
contact email	lpvaites@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011877

PRIDE project URI

• PRIDE
  1. PXD011877
     1. Label: PRIDE project
     2. Name: Protein aggregation mediates protein complex stoichiometry in aneuploid cells, part 4