PXD011869

PXD011869 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic SILAC analysis of enteroids deleted for Hdac1 or Hdac2
Description	We have exploited organoid SILAC approaches that we have previously developed (A SILAC-Based Method for Quantitative Proteomic Analysis of Intestinal Organoids.- Gonneaud A, Jones C, Turgeon N, Lévesque D, Asselin C, Boudreau F, Boisvert FM. -Sci Rep. 2016 Nov 30;6:38195. doi: 10.1038/srep38195) to investigate proteomic changes after deletion of epigenetic eraser genes Hdac1 and Hdac2 in enteroids. Both HDAC1 and HDAC2 are epigenetic erasers that drive specific and redundant gene expression patterns, in part by removing acetyl groups on histones. Deletion of these Hdac in intestinal epithelial cell (IEC) in vivo alters intestinal homeostasis, dependent on the Hdac deleted and the level of expression of both. To determine the intrinsic specific IEC function of HDAC1 and HDAC2, we have performed transcriptomic and quantitative proteomic approaches on enteroids deficient in Hdac1 or Hdac2. We have defined changes in both mRNA and protein expression patterns affecting IEC differentiation. We have identified IEC Hdac1- and Hdac2-dependent common as well as specific pathways and biological processes. These findings uncover unrecognized similarities and differences between Hdac1 and Hdac2 in IEC.
HostingRepository	PRIDE
AnnounceDate	2019-11-14
AnnouncementXML	Submission_2019-11-14_02:09:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexis Gonneaud
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-28 06:15:05	ID requested
⏵ 1	2019-11-14 02:14:30	announced

Gonneaud A, Turgeon N, Jones C, Couture C, L, é, vesque D, Boisvert FM, Boudreau F, Asselin C, HDAC1 and HDAC2 independently regulate common and specific intrinsic responses in murine enteroids. Sci Rep, 9(1):5363(2019) [pubmed]

submitter keyword: SILAC, Intestin, HDAC1 , HDAC2, enteroid

Claude Asselin
contact affiliation	Université de Sherbrooke
contact email	Claude.asselin@usherbrooke.ca
lab head
Alexis Gonneaud
contact affiliation	Université de Sherbrooke
contact email	alexis.gonneaud@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011869

PRIDE project URI

• PRIDE
  1. PXD011869
     1. Label: PRIDE project
     2. Name: Quantitative proteomic SILAC analysis of enteroids deleted for Hdac1 or Hdac2