PXD011865

PXD011865 is an original dataset announced via ProteomeXchange.

Title	SFPQ interacted proteins in HEK293T nucleus and cytoplasm
Description	Splicing factor proline and glutamine rich (SFPQ), DNA- and RNA binding protein, is crucial in various nuclear processes, including paraspeckle formation, miRNA synthesis and specially in transcription regulation. In addition, SFPQ play a role in the innate immune response to viruses, including DNA and RNA viruses. However, the connections between SFPQ and EMCV infection remain unclear. Here we report that the SFPQ is essential for EMCV replication. Depletion of SFPQ impairs EMCV production, while forced expression of SFPQ could promote viral replication. Mechanistically, EMCV inhibited viral RNA-mediated type I IFN and IL6 production to eliminate host antiviral immune responses. Cellular SFPQ was cleaved by the EMCV proteinase then entered the cytoplasm and interacted with other ribosomal proteins to facilitate its internal ribosome entry site (IRES)-dependent translation. Moreover, loss of SFPQ may impress host translation related gene expression and thus facilitate the EMCV replication. Altogether, our work provides a possible target for resisting EMCV or EMCV-like virus’s infection.
HostingRepository	PRIDE
AnnounceDate	2019-01-10
AnnouncementXML	Submission_2019-01-09_23:41:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bin Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-28 03:17:09	ID requested
⏵ 1	2019-01-09 23:41:08	announced

Dataset with its publication pending

submitter keyword: SFPQ

HEK2893T

Nucleus

Cytoplasm

Feng Qian
contact affiliation	School of Life Sciences Fudan University
contact email	fengqian@fudan.edu.cn
lab head
Bin Zhou
contact affiliation	School of Life Sciences Fudan University
contact email	zhoubin1999fd@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011865
     1. Label: PRIDE project
     2. Name: SFPQ interacted proteins in HEK293T nucleus and cytoplasm