PXD011860

PXD011860 is an original dataset announced via ProteomeXchange.

Title	Eukaryotic translation is modulated by natural mRNA nucleotide derivatives
Description	RNA modifications are crucial factors for efficient protein synthesis. All classes of RNAs that are involved in translation are modified to different extents. Recently, mRNA modifications and their impact on gene regulation became a focus of interest because they can exert a variety of effects on the fate of mRNAs. mRNA modifications within coding sequences can either directly or indirectly interfere with protein synthesis. In order to investigate the role of various natural occurring modified nucleotides, we site-specifically introduced them into the coding sequence of reporter mRNAs and subsequently translated them in HEK293T cells. The analysis of the respective translation products revealed a strong position-dependent impact of RNA modifications on translation efficiency and accuracy. Whereas a single 5-methylcytosine (m5C) or pseudouridine (Ψ) did not reduce product yields, 1-methyladenosine (m1A) generally impeded the translation of the respective modified mRNA. An inhibitory effect of 2’O-methlyated nucleotides (Nm) and 6-methyladenosine (m6A) was strongly dependent on their position within the codon. Finally, we could not attribute any miscoding potential to the set of mRNA modifications tested in HEK293T cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:50:56.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Hoernes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-28 00:35:20	ID requested
1	2019-01-30 04:08:10	announced
⏵ 2	2024-10-22 04:51:02	announced	2024-10-22: Updated project metadata.

10.3390/genes10020084;

Hoernes TP, Heimd, ö, rfer D, K, ö, stner D, Faserl K, Nu, ß, baumer F, Plangger R, Kreutz C, Lindner H, Erlacher MD, Eukaryotic Translation Elongation is Modulated by Single Natural Nucleotide Derivatives in the Coding Sequences of mRNAs. Genes (Basel), 10(2):(2019) [pubmed]

curator keyword: Biological

submitter keyword: translation elongation, ribosome, decoding,biological, nucleotide modifications

Matthias David Erlacher
contact affiliation	Medical University of Innsbruck - Biocenter, Division of Genomics & RNomics
contact email	matthias.erlacher@i-med.ac.at
lab head
Thomas Hoernes
contact affiliation	Medical University of Innsbruck - Biocenter, Division of Genomics & RNomics
contact email	thomas.hoernes@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011860
     1. Label: PRIDE project
     2. Name: Eukaryotic translation is modulated by natural mRNA nucleotide derivatives