PXD011856

PXD011856 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomics analysis of nuclear envelope, cytoplasmic membrane and nuclear contents fractions isolated from mesenchymal stem cells, adipocytes and myocytes
Description	We used shotgun proteomics to analyze three subcellular fractions isolated from mesenchymal stem cells and from differentiated adipocytes and myocytes: nuclear envelopes (NEs), cytoplasmic membranes and nuclear contents. The proteomics data were analyzed by a NSAF-based scoring system to calculate relative protein enrichment in the NE fraction. High NE enrichment scores were obtained for most well-characterized NE proteins. High scores also were seen for a number of new proteins not known to be concentrated at the NE, and we validated that five of these are substantially concentrated at the NE of multiple cell types. Our datasets can be useful for identifying additional NE-concentrated proteins in the future.
HostingRepository	MassIVE
AnnounceDate	2018-11-27
AnnouncementXML	Submission_2018-11-27_10:37:06.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salvador Martínez de Bartolomé
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-27 09:33:15	ID requested
⏵ 1	2018-11-27 10:37:07	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: shotgun proteomics, subcellular fractionation, nuclear envelope, endoplasmic reticulum, nuclear lamina, transmembrane protein, chromatin

submitter keyword: shotgun proteomics, subcellular fractionation, nuclear envelope, endoplasmic reticulum, nuclear lamina, transmembrane protein, chromatin

Contact List

Larry Gerace
contact affiliation	The Scripps Research Institute
contact email	lgerace@scripps.edu
lab head
John R. Yates III
contact affiliation	The Scripps Research Institute
contact email	jyates@scripps.edu
lab head
Salvador Martínez de Bartolomé
contact affiliation	The Scripps Research Institute
contact email	salvador@scripps.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000083166