PXD011855

PXD011855 is an original dataset announced via ProteomeXchange.

Title	Identification of a chondroitin sulfate linkage region trisaccharide
Description	Here, using LC-MS/MS, we describe for the first time a linkage region trisaccharide comprising [-GlcUA-Gal-Xyl-O-] as an alternative point of entry for glycosaminoglycan (GAG) biosynthesis. The trisaccharide was identified in the proteoglycan bikunin from urine of human healthy donors present as a disulfated pentasaccharide, [deltaHexUA-GalNAc(S)-GlcUA-Gal(S)-Xyl-O-] after chondroitinase ABC degradation. Furthermore, it was identified in chondroitin sulfate primed on xylosides isolated from human cell lines, present as the corresponding disulfated pentasaccharide after chondroitinase ABC degradation.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-03-26_05:45:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Egor Vorontsov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-27 08:37:55	ID requested
1	2019-02-25 05:08:18	announced
⏵ 2	2019-03-26 05:45:51	announced	Updated publication reference for PubMed record(s): 30824935.

Persson A, Nilsson J, Vorontsov E, Noborn F, Larson G, Identification of a non-canonical chondroitin sulfate linkage region trisaccharide. Glycobiology, 29(5):366-371(2019) [pubmed]

submitter keyword: LC-MS, Orbitrap, HCD, negative ionisation mode, glycosaminoglycan, bikunin, chondroitin sulfate

Göran Larson
contact affiliation	Professor, Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden
contact email	goran.larson@clinchem.gu.se
lab head
Egor Vorontsov
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact email	yegor.msu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD011855

PRIDE project URI

• PRIDE
  1. PXD011855
     1. Label: PRIDE project
     2. Name: Identification of a chondroitin sulfate linkage region trisaccharide