PXD011854

PXD011854 is an original dataset announced via ProteomeXchange.

Title	A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program
Description	Several studies have identified a specific metabolic program that is associated with the process of epithelial-mesenchymal transition (EMT). Whereas much is known about the association between glucose metabolism and EMT, the contribution of lipid metabolism is not still completely understood. Here, we studied epithelial and mesenchymal breast cancer cells by proteomic and lipidomic approaches and identified significant differences that characterised these models concerning specific metabolic enzymes and metabolites including fatty acids and phospholipids. Higher levels of monounsaturated fatty acids together with increased expression of enzymes of de novo fatty acid synthesis is the distinct signature of epithelial with respect to mesenchymal cells that, on the contrary, show reduced lipogenesis, higher polyunsaturated fatty acids level and increased expression of genes involved in the triglyceride (TAG) synthesis and lipid droplets formation. In the mesenchymal model, the diacylglycerol acyltransferase (DGAT)-1 appears to be the major enzyme involved in TAG synthesis and inhibition of DGAT1, but not DGAT2, drastically reduces the incorporation of labeled palmitate into TAG. Moreover, knockdown of β-catenin demonstrated that this metabolic phenotype in under the control of a network of transcriptional factors and that β-catenin has a specific role in the regulation of lipid metabolism in mesenchymal cells.
HostingRepository	PRIDE
AnnounceDate	2019-01-02
AnnouncementXML	Submission_2019-01-02_02:06:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Franck
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-27 08:23:44	ID requested
⏵ 1	2019-01-02 02:06:02	announced

Giudetti AM, De Domenico S, Ragusa A, Lunetti P, Gaballo A, Franck J, Simeone P, Nicolardi G, De Nuccio F, Santino A, Capobianco L, Lanuti P, Fournier I, Salzet M, Maffia M, Vergara D, A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program. Biochim Biophys Acta Mol Cell Biol Lipids, 1864(3):344-357(2019) [pubmed]

curator keyword: Biological

submitter keyword: Epithelial Mesenchymal Transition, Metabolism, Mass spectrometry, Lipids, β-catenin

daniele vergara
contact affiliation	University of Salento
contact email	daniele.vergara@unisalento.it
lab head
Julien Franck
contact affiliation	PRISM-INSERM U1192
contact email	julien.franck@univ-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011854

PRIDE project URI

• PRIDE
  1. PXD011854
     1. Label: PRIDE project
     2. Name: A specific lipid metabolic profile is associated with the epithelial mesenchymal transition program