PXD011849

PXD011849 is an original dataset announced via ProteomeXchange.

Title	Role of SurA, BamB, BamC and HlpA for shaping the outer membrane protein composition of Pseudomonas aeruginosa
Description	Pseudomonas aeruginosa (Pa) is one of the main causative agents of nosocomial infections and the spread of multidrug-resistant strains is rising. The outer membrane composition of Pa restricts antibiotic entry and determines virulence. For efficient outer membrane protein biogenesis, the BAM complex and chaperones like Skp and SurA are crucial. Deletion mutants of bamB, bamC and the skp homolog hlpA as well as a conditional mutant of surA were investigated. The most profound effects were associated with a lack of SurA, characterized by increased membrane permeability, enhanced sensitivity to antibiotic treatment and attenuation of virulence in a Galleria mellonella infection model. Strikingly, the conditional deletion of surA in a multidrug-resistant bloodstream isolate re-sensitized the strain to antibiotic treatment. Mass spectrometry revealed striking alterations in the outer membrane composition. Thus, SurA of Pa is important for the insertion of many porins, type V secretion systems, TonB-dependent receptors, proteins involved in LPS transport and BAM complex components. Therefore, SurA of Pa serves as a promising target for developing a drug that shows antiinfective activity and sensitizes multidrug-resistant strains to antibiotics.
HostingRepository	PRIDE
AnnounceDate	2019-03-12
AnnouncementXML	Submission_2019-03-11_17:49:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Nalpas
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-27 07:35:28	ID requested
⏵ 1	2019-03-11 17:49:24	announced

Klein K, Sonnabend MS, Frank L, Leibiger K, Franz-Wachtel M, Macek B, Trunk T, Leo JC, Autenrieth IB, Sch, ü, tz M, Bohn E, . Front Microbiol, 10():100(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Pseudomonas aeruginosa, outer membrane protein biogenesis, bacterial infection, cell surface molecules, SurA, BamB, BamC, HlpA

Erwin Bohn
contact affiliation	Interfakultäres Institut für Mikrobiologie und Infektionsmedizin Tübingen (IMIT) Institut für Medizinische Mikrobiologie und Hygiene Universität Tübingen Tübingen Germany
contact email	Erwin.bohn@med.uni-tuebingen.de
lab head
Nicolas Nalpas
contact affiliation	Tuebingen University
contact email	nalpas.nicolas@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD011849

PRIDE project URI

• PRIDE
  1. PXD011849
     1. Label: PRIDE project
     2. Name: Role of SurA, BamB, BamC and HlpA for shaping the outer membrane protein composition of Pseudomonas aeruginosa