PXD011844

PXD011844 is an original dataset announced via ProteomeXchange.

Title	Human osteoblasts quantitative proteomic analysis after cocultivation with endothelial cells
Description	We previously described that the coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves the differentiation of both cell types, leading to the formation of functional blood vessels and enhanced bone regeneration. The objective of this study was to further delineate the multifaceted interactions between both cell types. To investigate the proteome of hOBs after cocultivation with HUVECs we used stable isotope labeling by amino acids in cell culture
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2018-12-04_04:24:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oscar Winninger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-27 03:48:29	ID requested
1	2018-12-03 06:05:59	announced
⏵ 2	2018-12-04 04:25:00	announced	Updated project metadata.

Simunovic F, Winninger O, Strassburg S, Koch HG, Finkenzeller G, Stark GB, Lampert FM, Increased differentiation and production of extracellular matrix components of primary human osteoblasts after cocultivation with endothelial cells: A quantitative proteomics approach. J Cell Biochem, 120(1):396-404(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: angiogenesis, bone tissue engineering, human umbilical vein endothelial cells (HUVECs), primary human osteoblasts (hOBs), quantitative proteomics, stable isotope labeling by amino acids in cell culture (SILAC)

Günter Finkenzeller
contact affiliation	Chief, Section of Research and Tissue Engineering, Department of Plastic Surgery, University Medical Center Freiburg, Germany
contact email	guenter.finkenzeller@uniklinik-freiburg.de
lab head
Oscar Winninger
contact affiliation	University Medical Center Freiburg, Hand and Plastic Surgery
contact email	oscar.winninger@uniklinik-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/12/PXD011844

PRIDE project URI

• PRIDE
  1. PXD011844
     1. Label: PRIDE project
     2. Name: Human osteoblasts quantitative proteomic analysis after cocultivation with endothelial cells