PXD011825

PXD011825 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of TMV and TMV 24A+UPD infected N. benthamiana plants by iTRAQ reveal differences in stress signalling, cell death and response to intracellular iron
Description	TMV was mutated by introducing different lengths of internal poly (A) tract in front of the upstream pseudo knotted domain .Twenty four nucleotides were inserted into the TMV genome, this mutant is designated TMV 24A +UPD TMV 24+UPD shows more severe leaf necrosis and stem collapse, leading to plant death within a few days. The difference in rate of virus replication and plant death may be because the poly (A) tract introduced into TMV may interact with UPD and affect its interaction with host factors. To understand TMV 24A+UPD interaction with the host and the events leading to the altered pathogenesis, we opted to evaluate its proteome profile and compare it to TMV using Isobaric tag for relative and absolute quantitation( iTRAQ).
HostingRepository	PRIDE
AnnounceDate	2019-10-18
AnnouncementXML	Submission_2019-10-17_18:48:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mercy Macharia
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	methylthiolated residue; acetylated residue; monohydroxylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-26 02:20:08	ID requested
⏵ 1	2019-10-17 18:48:18	announced

Dataset with its publication pending

submitter keyword: TMV, TMV 24A+UPD

Prof. Wong Sek Man
contact affiliation	Department of Biological Science, National University of Singapore Singapore
contact email	dbswsm@nus.edu.sg
lab head
Mercy Macharia
contact affiliation	National University of Singapore
contact email	macekenya@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011825
     1. Label: PRIDE project
     2. Name: Proteomic analysis of TMV and TMV 24A+UPD infected N. benthamiana plants by iTRAQ reveal differences in stress signalling, cell death and response to intracellular iron