PXD011817

PXD011817 is an original dataset announced via ProteomeXchange.

Title	Protein composition of the extracellular matrix in idiopathic germ cell aplasia
Description	The somatic microenvironment supports spermatogonial stem cell differentiation into sperm. Extracellular matrix (ECM) plays multiple roles in the stem cell niche, including self-renewal, proliferation, differentiation and survival of spermatogonial cells. The pathophysiology of male infertility might be representative of a progressive degenerative process of the testicular tissue, including ECM, rather than a defective genetic background, thus outlining the existence of chronic etiological agents/pathways. In this context, we sought to identify potential causative factors responsible for a number of modifications of the testicular somatic microenvironment associated with idiopathic germ cell aplasia in human beings. Proteomic analysis of the decellularized ECM was performed to study testis parenchyma from 10 idiopathic non-obstructive azoospermic (iNOA) men, dichotomized according to positive sperm retrieval versus germ cell aplasia. Germ cell aplasia was characterized by an increased nuclear distribution of the retinoic acid receptor  in Sertoli cells which was associated with decreased expression of the ECM markers, Nidogen-2 and Heparan sulfate proteoglycan-2. Decreased levels of the interstitial matrisome associated Factor IX and its regulator VKORC1 were instead coupled with decreased signaling of vitamin K in Leydig cells. This study identified pathogenetic signature of the somatic testicular microenvironment and provide mechanistic insights into the molecular determinants of human idiopathic germ cell aplasia.
HostingRepository	PRIDE
AnnounceDate	2019-01-07
AnnouncementXML	Submission_2019-05-27_02:03:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011817
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Annapaola Andolfo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-23 09:07:49	ID requested
1	2019-01-07 07:42:17	announced
⏵ 2	2019-05-27 02:03:35	announced	Updated publication reference for PubMed record(s): 30929729.

Alfano M, Pederzoli F, Locatelli I, Ippolito S, Longhi E, Zerbi P, Ferrari M, Brendolan A, Montorsi F, Drago D, Andolfo A, Nebuloni M, Salonia A, Impaired testicular signaling of vitamin A and vitamin K contributes to the aberrant composition of the extracellular matrix in idiopathic germ cell aplasia. Fertil Steril, 111(4):687-698(2019) [pubmed]

submitter keyword: Extracellular matrix

male infertility

label-free quantitative proteomics

Massimo Alfano
contact affiliation	Urological Research Institute DiBit2, Scientific Institute San Raffaele Via Olgettina 60, 20132 Milan - Italy Tel: +39-02-2643.6351; Fax. +39-02-2643.5659
contact email	alfano.massimo@hsr.it
lab head
Annapaola Andolfo
contact affiliation	Ospedale San Raffaele
contact email	andolfo.annapaola@hsr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD011817

PRIDE project URI

• PRIDE
  1. PXD011817
     1. Label: PRIDE project
     2. Name: Protein composition of the extracellular matrix in idiopathic germ cell aplasia