PXD011811

PXD011811 is an original dataset announced via ProteomeXchange.

Title	The biomolecular characterisation of an Early Neolithic finger ring from Syltholm, Denmark
Description	We present the analysis of an osseous finger ring from an early Neolithic context in Denmark. To characterise the artefact and identify the raw material used for its manufacture, we performed micro-computed tomography (Micro CT) scanning, zooarchaeology by mass spectrometry (ZooMS) peptide mass fingerprinting, as well as protein sequencing by liquid chromatography tandem mass spectrometry (LC-MS/MS). We found that the ring was made from long bone or antler due to the presence of osteons (Haversian canals). Subsequent ZooMS analysis of the collagen present indicated that it was made from either elk (Alces alces) or red deer (Cervus elaphus) material. We then used LC-MS/MS analysis to refine our species identification, confirming that the ring was made from red deer, and to examine other proteins present. This study demonstrates the potential of ancient proteomics for species identification of prehistoric artefacts made from osseous material.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:50:35.890.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meaghan Mackie
SpeciesList	scientific name: Cervus elaphus (Red deer); NCBI TaxID: 9860;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-23 06:16:41	ID requested
1	2020-01-21 08:08:40	announced
2	2020-02-17 03:00:40	announced	2020-02-17: Updated publication reference for DOI(s): 10.1098/rsos.191172.
⏵ 3	2023-11-14 08:50:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: collagen

LC-MS/MS

mass spectrometry

palaeoproteomics

zooarchaeology

ZooMS

Hannes Schroeder
contact affiliation	Section for Evolutionary Genomics, University of Copenhagen, Øster Voldgade 5-7, 1350 Copenhagen, Denmark.
contact email	hschroeder@bio.ku.dk
lab head
Meaghan Mackie
contact affiliation	University of Copenhagen
contact email	meaghan@palaeome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD011811

PRIDE project URI

• PRIDE
  1. PXD011811
     1. Label: PRIDE project
     2. Name: The biomolecular characterisation of an Early Neolithic finger ring from Syltholm, Denmark