PXD011805 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mycobacterium smegtatis phosphoproteomics LC-MS/MS |
Description | Protein phosphatase PstP is conserved throughout the Actinobacteria in a genetic locus related to cell wall synthesis and cell division. In many Actinobacteria it is the sole annotated serine threonine protein phosphatase to counter the activity of multiple serine threonine protein kinases. We used transcriptional knockdown, electron microscopy and comparative phosphoproteomics to investigate the putative dual functions of PstP as a specific regulator of cell division and as a global regulator of protein phosphorylation. pstP knockdown in Mycobacterium smegmatis led to thickening of the cell wall and septum, changes in cell morphology, and eventual cell lysis. Comparative phosphoproteomics in the early stages of PstP depletion showed hyperphosphorylation of protein kinases and their substrates, confirming PstP as a negative regulator of kinase activity and global serine and threonine phosphorylation. Analysis of the 838 phosphorylation sites that changed significantly, suggested that PstP may have broad specificity, with preference for phosphothreonine, phosphosites near acidic residues, near the protein termini, and within membrane associated proteins. Increased phosphorylation of the activation loop of protein kinase B (PknB) and of the essential PknB substrate CwlM offer possible explanations for the essentiality of pstP and the cell wall defects when PstP was depleted. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-12 |
AnnouncementXML | Submission_2019-11-12_15:57:18.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Helen O'Hare |
SpeciesList | scientific name: Mycolicibacterium smegmatis MKD8; NCBI TaxID: 1214915; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-23 01:20:44 | ID requested | |
⏵ 1 | 2019-11-12 15:57:20 | announced | |
Publication List
Iswahyudi, Mukamolova GV, Straatman-Iwanowska AA, Allcock N, Ajuh P, Turapov O, O'Hare HM, Mycobacterial phosphatase PstP regulates global serine threonine phosphorylation and cell division. Sci Rep, 9(1):8337(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: bacterial protein phosphatase, bacterial signal transduction, phosphoproteomics, cell division, actinobacteria, mycobacteria, Mycobacterium smegmatis |
Contact List
Dr Helen O'Hare |
contact affiliation | University of Leicester, Department of Infection, Immunity & Inflammation and Molecular and Cell Biology, United Kingdom |
contact email | hmo7@le.ac.uk |
lab head | |
Helen O'Hare |
contact affiliation | University of Leicester |
contact email | hmo7@le.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011805
- Label: PRIDE project
- Name: Mycobacterium smegtatis phosphoproteomics LC-MS/MS