PXD011802

PXD011802 is an original dataset announced via ProteomeXchange.

Title	Makorin 1 controls embryonic patterning by alleviating Bruno-mediated repression of oskar translation
Description	Makorins are an evolutionary conserved family of proteins that contain C3H-type zinc finger modules and a RING E3 ubiquitin ligase domain. Previous analysis indicated a maternal role for Makorin 1 (Mkrn1) in Drosophila embryonic patterning and germ cell specification, but the underlying mechanism has remained elusive. Here, we show that Mkrn1 is specifically required for translational activation of oskar, which encodes a critical regulator of axis specification and germ plasm assembly. We demonstrate that Mkrn1 interacts with poly(A) binding protein (pAbp) and specifically binds osk 3’ UTR adjacent to A-rich sequences. The binding of Mkrn1 to osk 3’UTR occurs in a region that overlaps with Bruno responsive elements (BRE), previously shown to have a dual role in regulating osk translation. We observe an increased association of the translational repressor Bruno (Bru) with osk mRNA upon depletion of Mkrn1, implying that both proteins compete with each other for osk binding. Consistently, reducing Bru dosage is sufficient to partially rescue osk translation and the embryonic lethality associated with Mkrn1 alteration. Thus, we conclude that Mkrn1 controls embryonic patterning and germ cell formation by specifically activating osk translation via displacing Bru from its 3’ UTR
HostingRepository	PRIDE
AnnounceDate	2020-01-29
AnnouncementXML	Submission_2020-01-29_03:57:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Annabelle Dold
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-22 23:43:50	ID requested
⏵ 1	2020-01-29 03:57:46	announced

Dold A, Han H, Liu N, Hildebrandt A, Br, ü, ggemann M, R, ü, ckl, é C, H, ä, nel H, Busch A, Beli P, Zarnack K, K, ö, nig J, Roignant JY, Lasko P, Makorin 1 controls embryonic patterning by alleviating Bruno1-mediated repression of oskar translation. PLoS Genet, 16(1):e1008581(2020) [pubmed]

curator keyword: Biological

submitter keyword: polyadenylation, oogenesis, germ cell specification, pole plasm, Mkrn1, Drosophila

Jean-Yves Roignant
contact affiliation	RNA epigenetics, Institute of Molecular Biology (IMB), Mainz, Germany
contact email	j.roignant@imb-mainz.de
lab head
Annabelle Dold
contact affiliation	Institute of Molecular Biology Mainz
contact email	a.dold@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD011802

PRIDE project URI

• PRIDE
  1. PXD011802
     1. Label: PRIDE project
     2. Name: Makorin 1 controls embryonic patterning by alleviating Bruno-mediated repression of oskar translation