PXD011797

PXD011797 is an original dataset announced via ProteomeXchange.

Title	Global Enrichment of Low-Abundance Peptide Hormones in Plasma for Discovery and Quantitation
Description	We describe a method for global enrichment of active peptide hormones from blood plasma for both new hormone discovery and quantitative analysis. To extend the dynamic range of plasma proteomics, we employed high-resolution size exclusion chromatography to purify the peptide hormones and a powerful precipitation method to dissociate and remove bulk proteins prior to separation. Combining this method with high-sensitivity proteomic analysis, we characterized hormone abundance changes after intermittent fasting in humans.
HostingRepository	PRIDE
AnnounceDate	2019-07-16
AnnouncementXML	Submission_2019-07-18_00:47:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Larance
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-22 01:58:19	ID requested
1	2019-07-16 01:55:44	announced
⏵ 2	2019-07-18 00:55:06	announced	Updated publication reference for PubMed record(s): 31308252.

Harney DJ, Hutchison AT, Su Z, Hatchwell L, Heilbronn LK, Hocking S, James DE, Larance M, Small-protein Enrichment Assay Enables the Rapid, Unbiased Analysis of Over 100 Low Abundance Factors from Human Plasma. Mol Cell Proteomics, 18(9):1899-1915(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: human

plasma

intermittent fasting

peptide hormone

insulin

hepcidin

GLP-1

IGF-1.

Mark Colin Larance
contact affiliation	School of Life and Environmental Sciences, Charles Perkins Centre, Larance Lab, University of Sydney, Australia.
contact email	mark.larance@sydney.edu.au
lab head
Mark Larance
contact affiliation	The University of Sydney
contact email	mark.larance@sydney.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011797

PRIDE project URI

• PRIDE
  1. PXD011797
     1. Label: PRIDE project
     2. Name: Global Enrichment of Low-Abundance Peptide Hormones in Plasma for Discovery and Quantitation