PXD011792

PXD011792 is an original dataset announced via ProteomeXchange.

Title	In vivo proteome of Pseudomonas aeruginosa in airways of cystic fibrosis patients
Description	Chronic airway infection with P. aeruginosa (PA) is a hallmark of cystic fibrosis (CF) disease. The mechanisms producing PA persistence in CF therapies remain poorly understood. To gain insight on PA physiology in patient airways and better understand how in vivo bacterial functioning differs from in vitro conditions, we investigated the in vivo proteomes of PA in 35 sputum samples from 11 CF patients. We developed a novel bacterial-enrichment method enabling improved identification of PA proteome with CF sputum samples. The in vivo PA proteomes were compared with the proteomes of ex vivo-grown PA populations from the same patient sample. We detected 1528 PA proteins (encoded by 1458 core genes and 70 accessory genes) that were expressed in CF airways, of which 1178 proteins were commonly identified with the ex vivo-grown PA populations. Label-free quantitation and proteome comparison revealed the in vivo up-regulation of siderophore TonB-dependent receptors, remodeling in central carbon metabolism including glyoxylate cycle and lactate utilization, and alginate overproduction. Knowledge of these in vivo proteome differences or others derived using the presented methodology could lead to future treatment strategies aimed at altering PA physiology in vivo to compromise infectivity or improve antibiotic efficacy.
HostingRepository	PRIDE
AnnounceDate	2019-05-09
AnnouncementXML	Submission_2019-05-09_00:15:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xia Wu
SpeciesList	scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ FT; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-22 01:14:35	ID requested
⏵ 1	2019-05-09 00:15:40	announced

Wu X, Siehnel RJ, Garudathri J, Staudinger BJ, Hisert KB, Ozer EA, Hauser AR, Eng JK, Manoil C, Singh PK, Bruce JE, In Vivo Proteome of Pseudomonas aeruginosa in Airways of Cystic Fibrosis Patients. J Proteome Res, 18(6):2601-2612(2019) [pubmed]

curator keyword: Metaproteomics, Biomedical

submitter keyword: Pseudomonas aeruginosa, cystic fibrosis, quantitative proteomics, bacterial population proteomes

James E. Bruce
contact affiliation	University of Washington Department of Genome Sciences
contact email	jimbruce@uw.edu
lab head
Xia Wu
contact affiliation	University of Washington
contact email	xiawu2@uw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011792
     1. Label: PRIDE project
     2. Name: In vivo proteome of Pseudomonas aeruginosa in airways of cystic fibrosis patients