PXD011782

PXD011782 is an original dataset announced via ProteomeXchange.

Title	A mass spectrometry survey of chromatin-associated proteins in pluripotency and early lineage commitment
Description	Pluripotent Embryonic Stem Cells (ESCs) can be captured in vitro in different states, ranging from unrestricted ‘naïve’ to more developmentally constrained ‘primed’ pluripotency. Complexes involved in epigenetic regulation and key transcription factors have been shown to be involved in specifying these distinct states. In this study, we use proteomic profiling of the chromatin landscape in naive pluripotent ESCs, Epistem cells (EpiSCs) and early differentiated ESCs to survey the chromatin in naïve and primed pluripotency and during differentiation. We provide a comprehensive overview of epigenetic complexes situated on the chromatin and identify proteins associated with the maintenance and loss of pluripotency. The findings presented here indicate major compositional alterations of epigenetic complexes starting from ESC priming onwards. Our results contribute to the understanding of ESC differentiation and provide a framework for future studies of lineage commitment of ESCs.
HostingRepository	PRIDE
AnnounceDate	2019-07-04
AnnouncementXML	Submission_2019-07-04_04:25:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guido van Mierlo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-21 07:53:04	ID requested
⏵ 1	2019-07-04 04:33:06	announced

van Mierlo G, Wester RA, Marks H, A Mass Spectrometry Survey of Chromatin-Associated Proteins in Pluripotency and Early Lineage Commitment. Proteomics, 19(14):e1900047(2019) [pubmed]

submitter keyword: Naïve Pluripotency, Primed Pluripotency, Differentiation, Chromatin, Mouse, LC-MS

Hendrik Marks
contact affiliation	Molecular Biology, Radboud Institute for Molecular Life Sciences (RIMLS), Nijmegen, the Netherlands
contact email	h.marks@ncmls.ru.nl
lab head
Guido van Mierlo
contact affiliation	Radboud University
contact email	guidovanmierlo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD011782

PRIDE project URI

• PRIDE
  1. PXD011782
     1. Label: PRIDE project
     2. Name: A mass spectrometry survey of chromatin-associated proteins in pluripotency and early lineage commitment