PXD011776

PXD011776 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of Serratia marcescens (ATCC13880) by high-resolution mass spectrometry
Description	Serratia marcescens (S. marcescens), an opportunistic human pathogen, has been identified as a major cause of nosocomial infection and outbreaks. The purpose of this analysis is to examine the S. marcescens (ATCC 13880) protein profile using a high resolution mass spectrometry (MS). S. marcescens ATCC 13880 strain was grown in Luria-Bertani broth and the protein extracted underwent trypsin digestion followed by simple reverse phase liquid chromatography fractionation. Peptide fractions were then analyzed using Orbitrap Fusion Mass Spectrometry and raw MS data was processed using Proteome Discoverer software. The proteomic study identified 2,541 unique protein groups, corresponding to approximately 54% of the measured protein-coding genes. Bioinformatics analysis of these identified proteins demonstrated their involvement in biological processes such as cell wall organization, caperone-mediated protein folding and ATP binding. To our knowledge, this is the first high-performance S.marcescens proteomics analysis (ATCC 13880). These novel observations provide a key baseline molecular profile of the S. marcescens proteome which will prove to be helpful for the future research in understanding the host-pathogen interactions during infection, elucidating the mechanism of multidrug resistance and for developing novel diagnostic markers or vaccine for the disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:27.099.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sharath R
SpeciesList	scientific name: Serratia marcescens subsp. marcescens ATCC 13880; NCBI TaxID: 911022;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-21 03:30:22	ID requested
1	2020-05-21 22:58:33	announced
⏵ 2	2024-10-22 05:07:34	announced	2024-10-22: Updated project metadata.

10.34172/bi.2020.15;

Gangadharappa BS, Rajashekarappa S, Sathe G, by high-resolution mass spectrometry. Bioimpacts, 10(2):123-135(2020) [pubmed]

submitter keyword: Antibiotic resistance, Gram negative bacteria,Serratia, LC-MS/MS, Orbitrap Fusion

Dr. Sharath R
contact affiliation	Dr. Sharath R Associate Professor Department of Food Technology, Davangere University Shivagangotri, Davanagere - 577007, Karnataka, India.
contact email	sharathsarathi@gmail.com
lab head
Sharath R
contact affiliation	Associate Professor, Department of Food Technology, Shivagangotri, Davangere University, Davangere-577007
contact email	sharathsarathi@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD011776

PRIDE project URI

• PRIDE
  1. PXD011776
     1. Label: PRIDE project
     2. Name: Proteomic profiling of Serratia marcescens (ATCC13880) by high-resolution mass spectrometry