PXD011767

PXD011767 is an original dataset announced via ProteomeXchange.

Title	EGR1 regulation in human bone marrow stromal cells
Description	Human bone marrow stromal cells (BMSCs) are key elements of the hematopoietic environment and they play a central role in bone and bone marrow physiology. However, how key BMSC functions are regulated is largely unknown. We analyzed the role of the immediate early response transcription factor EGR1 as key BMSC regulator and found that EGR1 was highly expressed in prospectively-isolated primary BMSCs, downregulated upon culture, and lower in non-CFU-F-containing CD45neg BM cells. Furthermore, EGR1 expression was lower in proliferative regenerating adult and fetal primary cells compared to adult steady-state BMSCs. Accordingly, EGR1 overexpression markedly decreased BMSC proliferation but considerably improved hematopoietic stroma support function as indicated by an increased production of transplantable CD34+CD90+ hematopoietic stem cells in expansion co-cultures. The improvement of BMSC stroma support function was mediated by increased expression of hematopoietic supporting genes, such as VCAM1 and CCL28. On the other hand, EGR1 knockdown increased ROS-mediated BMSC proliferation, and clearly reduced BMSC hematopoietic stroma support potential. These findings thus show that EGR1 is a key BMSC transcription factor with a dual role in regulating proliferation and hematopoietic stroma support function that is controlling a genetic program to coordinate the specific functions of BMSC in their different biological contexts.
HostingRepository	PRIDE
AnnounceDate	2019-06-27
AnnouncementXML	Submission_2019-08-06_08:06:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Hansson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2x(13)C; 4x(2)H labeled dimethylated residue; dimethylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-20 07:24:33	ID requested
1	2019-06-27 00:47:00	announced
⏵ 2	2019-08-06 08:06:42	announced	Updated publication reference for PubMed record(s): 31371413.

Li H, Lim HC, Zacharaki D, Xian X, Kenswil KJG, Br, ä, unig S, Raaijmakers MHGP, Woods NB, Hansson J, Scheding S, Early growth response 1 regulates hematopoietic support and proliferation in human primary bone marrow stromal cells. Haematologica, 105(5):1206-1215(2020) [pubmed]

curator keyword: Biological

submitter keyword: Human, bone marrow, stromal cells

Jenny Hansson
contact affiliation	Division of Molecular Hematology, Department of Laboratory Medicine, Lund Univsersity, Sweden
contact email	jenny.hansson@med.lu.se
lab head
Jenny Hansson
contact affiliation	Lund University
contact email	jehansson@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD011767

PRIDE project URI

• PRIDE
  1. PXD011767
     1. Label: PRIDE project
     2. Name: EGR1 regulation in human bone marrow stromal cells