PXD011763

PXD011763 is an original dataset announced via ProteomeXchange.

Title	Nitric oxide-dependent anaerobic ammonium oxidation
Description	Nitric oxide (NO) has several important functions in biology and atmospheric chemistry as a toxin, signaling molecule, ozone depleting agent and the precursor of the greenhouse gas nitrous oxide (N2O). Even though NO is a potent oxidant, and was available on earth earlier than oxygen, its direct use by microorganisms for growth was not demonstrated before. Using physiological experiments, metatranscriptomics and metaproteomics, here we show that anaerobic ammonium-oxidizing (anammox) bacterium Kuenenia stuttgartiensis grow by coupling ammonium oxidation to NO reduction, and produce only N2. Such a metabolism could have existed on early earth, and has implications in controlling N2O and NO emissions both from natural and manmade ecosystems, where anammox bacteria contribute significantly to N2 release to the atmosphere.
HostingRepository	PRIDE
AnnounceDate	2019-02-26
AnnouncementXML	Submission_2019-03-26_09:47:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hans Wessels
SpeciesList	scientific name: Candidatus Kuenenia stuttgartiensis; NCBI TaxID: 174633;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-20 05:16:20	ID requested
1	2019-02-26 00:16:08	announced
⏵ 2	2019-03-26 09:47:59	announced	Updated publication reference for PubMed record(s): 30886150.

Hu Z, Wessels HJCT, van Alen T, Jetten MSM, Kartal B, Nitric oxide-dependent anaerobic ammonium oxidation. Nat Commun, 10(1):1244(2019) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS/MS, Anammox, Kuenenia Stuttgartiensis

Alain J van Gool
contact affiliation	Translational Metabolic Laboratory, Department of Laboratory Medicine, Radboudumc
contact email	Alain.vanGool@radboudumc.nl
lab head
Hans Wessels
contact affiliation	Radboudumc
contact email	hans.wessels@radboudumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD011763

PRIDE project URI

• PRIDE
  1. PXD011763
     1. Label: PRIDE project
     2. Name: Nitric oxide-dependent anaerobic ammonium oxidation