PXD011758

PXD011758 is an original dataset announced via ProteomeXchange.

Title	Arhgef11 high and low salt kidney proteomic analysis
Description	Arhgef11 is a Rho guanine nucleotide exchange factor previously implicated in kidney injury in the Dahl salt-sensitive rat (SS-WT). Through exchange of GDP for GTP, Arhgef11 regulates cytoskeletal structure, function, and cell-cell contacts through a variety of stimuli (e.g., G-protein coupled receptors, growth factors, and shear stress). We studied the SS-Arhgef11-/- rat model at 4-12 weeks of age under low and high salt (0.3% or 2% NaCl). To better understand the molecular mechanisms associated with renal protection from loss of Arhgef11, MS/MS proteomics was performed on kidney from SS-Arhgef11-/- and SS-WT at week 12 (low-salt). At week 12, 1017 genes and 363 proteins were observed. In summary, in vivo phenotyping provides strong evidence that increased Arhgef11 expression in the Dahl S rat leads to actin cytoskeleton mediated changes in cell morphology and cell function (impaired reuptake filtered protein) that promote kidney injury, hypertension, and decline in kidney function.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_07:58:36.150.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elizabeth Flynn
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-20 01:47:56	ID requested
⏵ 1	2021-09-08 07:58:37	announced

Johnson AC, Wu W, Attipoe EM, Sasser JM, Taylor EB, Showmaker KC, Kyle PB, Lindsey ML, Garrett MR, in the Dahl Salt-Sensitive Rat Protects Against Hypertension-Induced Renal Injury. Hypertension, 75(4):1012-1024(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Arhgef11, high and low salt, kidney function

Elizabeth Flynn
contact affiliation	University of Mississippi Medical Center 2500 North State Street, G378 Jackson MS 39216-4505
contact email	EFlynn@umc.edu
lab head
Elizabeth Flynn
contact affiliation	University of Mississippi Medical Center
contact email	EFlynn@umc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011758
     1. Label: PRIDE project
     2. Name: Arhgef11 high and low salt kidney proteomic analysis