PXD011753

PXD011753 is an original dataset announced via ProteomeXchange.

Title	Hfq is involved in global protein expression and stress response in Bordetella pertussis
Description	In this study, we used gel-free nanoLC-MS/MS-based proteomics to compare the protein profile of B. pertussis wild type and an isogenic hfq defective mutant strain under control an iron limited conditions. We found that Hfq affects the abundance of 302 proteins, which represents 8% of the total B. pertussis coding sequence. The absence of Hfq induced changes in the abundance of proteins involved in metabolic pathways, stress response and virulence. Hfq was also found involved in B. pertussis adaptation to iron starvation, one of the main stresses this pathogen faces inside the host. Altogether, these results indicate that B. pertussis Hfq is involved in bacterial physiological processes as well as bacterial pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2020-02-18
AnnouncementXML	Submission_2020-02-18_07:53:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristin Surmann
SpeciesList	scientific name: Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251); NCBI TaxID: 257313;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-20 01:24:33	ID requested
⏵ 1	2020-02-18 07:53:18	announced

Alvarez Hayes J, Surmann K, Lamberti Y, Depke M, Dhople V, Blanc, á B, Ruiz E, Vecerek B, Schmidt F, V, ö, lker U, Rodriguez ME, Hfq modulates global protein pattern and stress response in Bordetella pertussis. J Proteomics, 211():103559(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: B. pertussis, Hfq, iron limitation, proteomics, stress response

Prof. Uwe Völker
contact affiliation	Department of Functional Genomics, Institute for Genetics and Functional Genomics, University Medicine Greifswald, Germany
contact email	voelker@uni-greifswald.de
lab head
Kristin Surmann
contact affiliation	University Medicine Greifswald
contact email	surmannk@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD011753

PRIDE project URI

• PRIDE
  1. PXD011753
     1. Label: PRIDE project
     2. Name: Hfq is involved in global protein expression and stress response in Bordetella pertussis