PXD011752

PXD011752 is an original dataset announced via ProteomeXchange.

Title	SERPA: Autoantibody profiling in POAG
Description	Glaucoma is the leading cause of irreversible blindness worldwide. An early diagnosis of the disease is crucial for treatment success. Mechanisms behind glaucoma pathogenesis are not completely understood, but disease related alterations in the serological autoantibody profile indicate an immunologic component. These changes in immunoreactivity may serve as potential biomarkers. Therefore, a serological proteome analysis (SERPA) approach was used to identify novel autoantibody biomarker candidates in primary open angle glaucoma. Autoantibodies in individual sera samples of glaucoma patients and control were analysed by serological proteome analysis (SERPA) using porcine trabecular meshwork (TM) tissue lysates as antigen source. Immunoblot spot intensities were computed by “Delta2D” (Decodon) and spots of interest were matched with preparative 2DE gels and identified by mass spectrometry to identify the potential autoantigens.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:50:27.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanessa Beutgen
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-20 01:22:26	ID requested
1	2019-02-21 03:25:54	announced
2	2019-03-25 02:52:20	announced	Updated publication reference for PubMed record(s): 30899261.
⏵ 3	2024-10-22 04:50:29	announced	2024-10-22: Updated project metadata.

Beutgen VM, Perumal N, Pfeiffer N, Grus FH, Autoantibody Biomarker Discovery in Primary Open Angle Glaucoma Using Serological Proteome Analysis (SERPA). Front Immunol, 10():381(2019) [pubmed]

10.3389/fimmu.2019.00381;

curator keyword: Biomedical

submitter keyword: autoantibodies

SERPA

immunoproteomics

glaucoma

POAG

Franz H. Grus
contact affiliation	Department of ophthalmology, experimental and translational ophthalmology, University medical center of the Johannes Gutenberg - University Mainz
contact email	grus@eye-research.org
lab head
Vanessa Beutgen
contact affiliation	Universitätsmedizin der Johannes Gutenberg - Universität Mainz
contact email	vbeutgen@eye-research.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD011752

PRIDE project URI

• PRIDE
  1. PXD011752
     1. Label: PRIDE project
     2. Name: SERPA: Autoantibody profiling in POAG