PXD011740

PXD011740 is an original dataset announced via ProteomeXchange.

Title	Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B
Description	Chondrocytes undergo changes to their protein translational capacity during osteoarthritis progression, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptomic level has not previously been performed. In this study we describe how the inflammatory cytokine IL-1B rapidly affects protein translation in the chondrocytic cell line SW1353. Using ribosome profiling we demonstrate that IL-1B induced altered translation of inflammatory-associated transcripts, as well as a number of ribosome-associated transcripts, through differential translation and the use of multiple open reading frames. Proteomic analtsis of the cell layer and conditioned media of these cells identified that proteins which were differentially translated were most readily detected in the secretome. We have produced combined ribosome profiling and proteomic datasets which provide a valuble resource in understanding the processes that are occoring during cytokine stimulation of chondrocytic cells.
HostingRepository	PRIDE
AnnounceDate	2019-09-27
AnnouncementXML	Submission_2019-09-27_06:09:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011740
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ben McDermott
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-19 08:24:07	ID requested
⏵ 1	2019-09-27 06:09:08	announced

McDermott BT, Peffers MJ, McDonagh B, Tew SR, . J Biol Chem, 294(35):13027-13039(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, chondrocytes, ribosome profiling, interleukin-1B, inflammation, secretion

Simon Tew
contact affiliation	Comparative Musculoskeletal Sciences Research Group Departmemt of Musculoskeletal Biology Institute of Ageing and Chronic Disease University of Liverpool
contact email	tew@liverpool.ac.uk
lab head
Ben McDermott
contact affiliation	University of Liverpool
contact email	benmcd@liverpool.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011740
     1. Label: PRIDE project
     2. Name: Translational regulation contributes to the secretory response of chondrocytic cells following exposure to Interleukin-1B