PXD011734

PXD011734 is an original dataset announced via ProteomeXchange.

Title	The puzzling construction of the conidial outer layer of Aspergillus fumigatus
Description	The outer layers of the fungal cell wall are the first barrier facing external challenges. In theinvasive morphotype of the airborne pathogen Aspergillus fumigatus, i.e. conidia, the outer cell wall consists of α-(1,3)-glucan, melanin and proteinaceous rodlets made up by the RodA hydrophobin. The hydrophobicity of the RodA rodlets facilitates air dispersal of conidia and progression within lungs. When conidia reach the alveolae, these are phagocytosed by host cells and killed upon germination, which requires melanin and rodlets elimination to occur. The dormant condition of the conidia protects them against activation of the host killing pathways. The outer cell wall of conidia is thus essential for maintaining the viability of the fungus. To study the role of α-(1,3)-glucan, melanin, and RodA rodlets in conidia protection, multiple mutants without two or the three major components of the outer layer were constructed and analyzed. Deletions led to the exposure of new molecules on the conidial surface. Multiple gene deletions did not always lead to logical additivity of the phenotypes. Unexpected compensatory cell wall surface modifications were indeed observed, such as the synthesis of the mycelial virulence factor galactosaminogalactan, the presence of chitin and glycoproteins or specific changes in permeability. In spite of significant modifications, sensitivity to killing by phagocytic host cells of the multiple mutants involving melanin changed little compared to the sensitive parent strain lacking melanin (ΔpksP), further indicating the importance of melanin in protecting conidia against killing by monocytes.
HostingRepository	PRIDE
AnnounceDate	2019-01-30
AnnouncementXML	Submission_2019-01-30_10:52:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magalie Duchateau
SpeciesList	scientific name: Aspergillus fumigatus CEA10; NCBI TaxID: 505235;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-19 08:15:53	ID requested
⏵ 1	2019-01-30 10:52:22	announced

Valsecchi I, Dupres V, Michel JP, Duchateau M, Matondo M, Chamilos G, Saveanu C, Guijarro JI, Aimanianda V, Lafont F, Latg, é JP, Beauvais A, The puzzling construction of the conidial outer layer of Aspergillus fumigatus. Cell Microbiol, 21(5):e12994(2019) [pubmed]

curator keyword: Biological

submitter keyword: Aspergillus fumigatus, conida, out layer

Jean-Paul Latgé
contact affiliation	Unité des Aspergillus Institut Pasteur 28, rue du Docteur Roux 75015 Paris
contact email	jplatge@pasteur.fr
lab head
Magalie Duchateau
contact affiliation	Institut Pasteur
contact email	magalie.duchateau@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011734
     1. Label: PRIDE project
     2. Name: The puzzling construction of the conidial outer layer of Aspergillus fumigatus