PXD011732

PXD011732 is an original dataset announced via ProteomeXchange.

Title	Profiling Protein Expression Levels and Genetically Variant Peptides in Human Hair From Different Body Sites
Description	Proteins of the human hair shaft contain a wealth of information about the coding regions of the person’s genome from whom the hair is derived. Commonly found at crime scenes, hair shafts may thus provide useful forensic evidence if the information they contain can be exploited. Present experiments show that hair shafts from four different anatomic sites are similarly useful in distinguishing individuals by protein profiling. However, the results demonstrated that protein profiles were dependent on anatomic site, indicating that a proper comparison requires matching the sites of origin. The differences in profile offer the prospect of determining the site of origin of hair by comparison with profiles of shafts from other anatomic sites. By contrast, the genetically variant peptides detected in the protein digests, that map to non-synonymous single nucleotide polymorphisms in subject DNA, were detectable regardless of the anatomic origin of the hair shafts. The resulting profiles of genetically variant peptides were more dependent on a subject’s genotype than on the anatomic origin of the hair shaft. Individual identification therefore can be based on peptide profiles regardless of body location. This study demonstrates the utility of proteomic analysis for increasing the forensic value of hair shaft evidence.
HostingRepository	PRIDE
AnnounceDate	2019-04-04
AnnouncementXML	Submission_2019-04-04_00:33:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Glendon Parker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-19 07:57:37	ID requested
⏵ 1	2019-04-04 00:33:06	announced

Milan JA, Wu PW, Salemi MR, Durbin-Johnson BP, Rocke DM, Phinney BS, Rice RH, Parker GJ, Comparison of protein expression levels and proteomically-inferred genotypes using human hair from different body sites. Forensic Sci Int Genet, 41():19-23(2019) [pubmed]

curator keyword: Biological

submitter keyword: Hair Shafts, Genetically Variant Peptides, Protein Profiling, Human Identification

Glendon J. Parker
contact affiliation	Department of Environmental Toxicology, University of California, Davis, USA
contact email	gjparker@ucdavis.edu
lab head
Glendon Parker
contact affiliation	UC Davis
contact email	gjparker@ucdavis.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD011732

PRIDE project URI

• PRIDE
  1. PXD011732
     1. Label: PRIDE project
     2. Name: Profiling Protein Expression Levels and Genetically Variant Peptides in Human Hair From Different Body Sites