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PXD011731

PXD011731 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDifferential modification of the C. elegans proteome in response to acute and chronic gamma radiation: link with reproduction decline
DescriptionEcosystems are chronically exposed to ionizing radiations. But environmental risk assessment of chronic exposure suffers from a lack of knowledge. Extrapolation of data from acute to chronic exposure is not always relevant, and can lead to uncertainties. In fact, effects could be different between the two irradiation modes, especially regarding reproduction endpoint, which is an ecologically relevant parameter . The free living nematode Caenorhabditis elegans is a particularly appropriate model organism to address this proteomic issues. With its fully sequenced genome and its short life cycle, C. elegans has been successfully used to study acute and chronic irradiation effects and their consequences on germline development and hatching. Results showed that a decrease of the number of progeny associated with a decrease of hatching success occurred from and above 30 Gy of acute irradiation.. In the present study, we decided to refine the understanding of molecular mechanisms of acute and chronic irradiation by a global proteome analysis. To do so, C. elegans were exposed to 3 common cumulated doses between acute and chronic exposure. These 3 doses, lower than the doses for which an effect on the reproduction was shown, were susceptible to allow us to find early and sensitive biomarkers of a reproduction decline. After exposure, global modification of the proteome expression was studied using a label free LC-MS/MS proteomic approach. Our objectives were to test the following hypotheses: (1) whether or not proteome expression varied with the dose, and with the irradiation mode; (2) if proteome expression modification was associated with effects on reproduction, with potential direct implications for ecological risk assessment.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:13:37.674.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuc Camoin
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-11-19 07:57:02ID requested
12020-10-15 06:12:59announced
22024-10-22 05:13:45announced2024-10-22: Updated project metadata.
Publication List
Dubois C, Pophillat M, Audebert S, Fourquet P, Lecomte C, Dubourg N, Galas S, Camoin L, Frelon S, Differential modification of the C. elegans proteome in response to acute and chronic gamma radiation: Link with reproduction decline. Sci Total Environ, 676():767-781(2019) [pubmed]
10.1016/j.scitotenv.2019.04.039;
Keyword List
curator keyword: Biomedical
submitter keyword: Gamma radiation, LC-MS,label-free quantification, Caenorhabditis elegans
Contact List
Luc Camoin
contact affiliationMarseille Proteomics, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes
contact emailluc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliationLife Sciences
contact emailluc.camoin@inserm.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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