PXD011731

PXD011731 is an original dataset announced via ProteomeXchange.

Title	Differential modification of the C. elegans proteome in response to acute and chronic gamma radiation: link with reproduction decline
Description	Ecosystems are chronically exposed to ionizing radiations. But environmental risk assessment of chronic exposure suffers from a lack of knowledge. Extrapolation of data from acute to chronic exposure is not always relevant, and can lead to uncertainties. In fact, effects could be different between the two irradiation modes, especially regarding reproduction endpoint, which is an ecologically relevant parameter . The free living nematode Caenorhabditis elegans is a particularly appropriate model organism to address this proteomic issues. With its fully sequenced genome and its short life cycle, C. elegans has been successfully used to study acute and chronic irradiation effects and their consequences on germline development and hatching. Results showed that a decrease of the number of progeny associated with a decrease of hatching success occurred from and above 30 Gy of acute irradiation.. In the present study, we decided to refine the understanding of molecular mechanisms of acute and chronic irradiation by a global proteome analysis. To do so, C. elegans were exposed to 3 common cumulated doses between acute and chronic exposure. These 3 doses, lower than the doses for which an effect on the reproduction was shown, were susceptible to allow us to find early and sensitive biomarkers of a reproduction decline. After exposure, global modification of the proteome expression was studied using a label free LC-MS/MS proteomic approach. Our objectives were to test the following hypotheses: (1) whether or not proteome expression varied with the dose, and with the irradiation mode; (2) if proteome expression modification was associated with effects on reproduction, with potential direct implications for ecological risk assessment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:13:37.674.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Camoin
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-19 07:57:02	ID requested
1	2020-10-15 06:12:59	announced
⏵ 2	2024-10-22 05:13:45	announced	2024-10-22: Updated project metadata.

Dubois C, Pophillat M, Audebert S, Fourquet P, Lecomte C, Dubourg N, Galas S, Camoin L, Frelon S, Differential modification of the C. elegans proteome in response to acute and chronic gamma radiation: Link with reproduction decline. Sci Total Environ, 676():767-781(2019) [pubmed]

10.1016/j.scitotenv.2019.04.039;

curator keyword: Biomedical

submitter keyword: Gamma radiation, LC-MS,label-free quantification, Caenorhabditis elegans

Luc Camoin
contact affiliation	Marseille Proteomics, Inserm UMR1068, CNRS UMR7258, Aix Marseille Université U105, Institut Paoli Calmettes
contact email	luc.camoin@inserm.fr
lab head
Luc Camoin
contact affiliation	Life Sciences
contact email	luc.camoin@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011731
     1. Label: PRIDE project
     2. Name: Differential modification of the C. elegans proteome in response to acute and chronic gamma radiation: link with reproduction decline